제품 > 항체 > 다중클론항체(pAb)

DNASE1 Rabbit pAb (A14531)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Mouse, Rat

ABclonal:Immunofluorescence - DNASE1 Rabbit pAb (A14531)

Immunofluorescence analysis of C6 cells using DNASE1 Rabbit pAb (A14531) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - DNASE1 Rabbit pAb (A14531)

Immunofluorescence analysis of NIH-3T3 cells using DNASE1 Rabbit pAb (A14531) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameDNASE1 Rabbit pAb
Catalog No.A14531
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the DNase family. This protein is stored in the zymogen granules of the nuclear envelope and functions by cleaving DNA in an endonucleolytic manner. At least six autosomal codominant alleles have been characterized, DNASE1*1 through DNASE1*6, and the sequence of DNASE1*2 represented in this record. Mutations in this gene have been associated with systemic lupus erythematosus (SLE), an autoimmune disease. A recombinant form of this protein is used to treat the one of the symptoms of cystic fibrosis by hydrolyzing the extracellular DNA in sputum and reducing its viscosity. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 30-235 of human DNASE1 (NP_005214.2).
SequenceIQTFGETKMSNATLVSYIVQILSRYDIALVQEVRDSHLTAVGKLLDNLNQDAPDTYHYVVSEPLGRNSYKERYLFVYRPDQVSAVDSYYYDDGCEPCGNDTFNREPAIVRFFSRFTEVREFAIVPLHAAPGDAVAEIDALYDVYLDVQEKWGLEDVMLMGDFNAGCSYVRPSQWSSIRLWTSPTFQWLIPDSADTTATPTHCAYDR
Gene ID
Swiss Prot
SynonymsDNL1; DRNI; DNASE1
Calculated MW31kDa
Observed MWRefer to Figures
ReactivityMouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samples
Cellular locationNucleus envelope, Secreted

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

    ABclonal:Immunofluorescence - DNASE1 Rabbit pAb (A14531)}

    Immunofluorescence - DNASE1 Rabbit pAb (A14531)

    Immunofluorescence analysis of C6 cells using DNASE1 Rabbit pAb (A14531) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - DNASE1 Rabbit pAb (A14531)}

    Immunofluorescence - DNASE1 Rabbit pAb (A14531)

    Immunofluorescence analysis of NIH-3T3 cells using DNASE1 Rabbit pAb (A14531) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

    ELISA 키트 (1)

    항체 (1)

    Secondary Antibodies (26)