제품 > 항체 > 다중클론항체(pAb)

DNTTIP2 Rabbit pAb (A16120)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - DNTTIP2 Rabbit pAb (A16120)

Western blot analysis of lysates from mouse pancreas, using DNTTIP2 Rabbit pAb (A16120) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunofluorescence - DNTTIP2 Rabbit pAb (A16120)

Immunofluorescence analysis of U-2 OS cells using DNTTIP2 Rabbit pAb (A16120) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameDNTTIP2 Rabbit pAb
Catalog No.A16120
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene is thought to be involved in chromatin remodeling and gene transcription. The encoded nuclear protein binds to and enhances the transcriptional activity of the estrogen receptor alpha, and also interacts with terminal deoxynucleotidyltransferase. The expression profile of this gene is a potential biomarker for chronic obstructive pulmonary disease.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human DNTTIP2 (NP_055412.2).
SequenceMVVTRSARAKASIQAASAESSGQKSFAANGIQAHPESSTGSDARTTAESQTTGKQSLIPRTPKARKRKSRTTGSLPKGTEPSTDGETSEAESNYSVSEHHDTILRVTRRRQILIACSPVSSVRKKPKVTPTKESYTEEIVSEAESHVSGISRIVLPTEKTTGARRSKAKSLTDPSQESHTEAISDAETSSSDISFSGIATRRTRSMQRKLKAQTEKKDSKIVPGNEKQIVGTPVNSEDSDTRQTSHLQARSLSEINKPNFYNNDFDDDFSHRSSENILTVHEQANVESLKETKQNCKDLD
Gene ID
Swiss Prot
SynonymsERBP; FCF2; TdIF2; HSU15552; LPTS-RP2; DNTTIP2
Calculated MW84kDa
Observed MW105kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesMouse pancreas
Cellular locationNucleus

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ABclonal:Western blot - DNTTIP2 Rabbit pAb (A16120)}

Western blot - DNTTIP2 Rabbit pAb (A16120)

Western blot analysis of lysates from mouse pancreas, using DNTTIP2 Rabbit pAb (A16120) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunofluorescence - DNTTIP2 Rabbit pAb (A16120)}

Immunofluorescence - DNTTIP2 Rabbit pAb (A16120)

Immunofluorescence analysis of U-2 OS cells using DNTTIP2 Rabbit pAb (A16120) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.