제품 > 항체 > 다중클론항체(pAb)

DREB1B Rabbit pAb (A21961)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Arabidopsis thaliana

ABclonal:Western blot - DREB1B Rabbit pAb (A21961)

Western blot analysis of extracts of various tissues from Arabidopsis thaliana, using DREB1B Rabbit pAb (A21961) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

Overview

Product nameDREB1B Rabbit pAb
Catalog No.A21961
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Transcriptional activator that binds to the DRE/CRT regulatory element and induces COR (cold-regulated) gene expression increasing plant freezing tolerance. It encodes a member of the DREB subfamily A-1 of ERF/AP2 transcription factor family (CBF1). The protein contains one AP2 domain. There are six members in this subfamily, including CBF1, CBF2, and CBF3. This gene is involved in response to low temperature and abscisic acid.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 50-150 of arabidopsis thaliana DREB1B (NP_567721.1).
SequenceGVRQRNSGKWVSEVREPNKKTRIWLGTFQTAEMAARAHDVAALALRGRSACLNFADSAWRLRIPESTCAKDIQKAAAEAALAFQDETCDTTTTNHGLDMEE
Gene ID
Swiss Prot
SynonymsATCBF1; C-repeat/DRE binding factor 1; DRE BINDING PROTEIN 1B; DREB1B; T30C3.11; TRANSCRIPTIONAL ACTIVATOR CBF1
Calculated MW24kDa
Observed MW25kDa
ReactivityArabidopsis thaliana
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesSeedling, Inflorescences
Cellular locationnucleus
    ABclonal:Western blot - DREB1B Rabbit pAb (A21961)}

    Western blot - DREB1B Rabbit pAb (A21961)

    Western blot analysis of extracts of various tissues from Arabidopsis thaliana, using DREB1B Rabbit pAb (A21961) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 30s.

    * For research use only. Not for therapeutic or diagnostic purposes.