제품 > 항체 > 다중클론항체(pAb)

DRG2 Rabbit pAb (A17476)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - DRG2 Rabbit pAb (A17476)

Western blot analysis of various lysates using DRG2 Rabbit pAb (A17476) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Immunofluorescence - DRG2 Rabbit pAb (A17476)

Immunofluorescence analysis of C6 cells using DRG2 Rabbit pAb (A17476) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - DRG2 Rabbit pAb (A17476)

Immunofluorescence analysis of L929 cells using DRG2 Rabbit pAb (A17476) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameDRG2 Rabbit pAb
Catalog No.A17476
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a GTP-binding protein known to function in the regulation of cell growth and differentiation. Read-through transcripts containing this gene and a downstream gene have been identified, but they are not thought to encode a fusion protein. This gene is located within the Smith-Magenis syndrome region on chromosome 17.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 150-260 of human DRG2 (NP_001379.1).
SequenceTKGEVQRSLLEKELESVGIRLNKHKPNIYFKPKKGGGISFNSTVTLTQCSEKLVQLILHEYKIFNAEVLFREDCSPDEFIDVIVGNRVYMPCLYVYNKIDQISMEEVDRLA
Gene ID
Swiss Prot
SynonymsDRG2
Calculated MW41kDa
Observed MW45kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesRat heart, Rat thymus
Cellular locationcytoplasm, cytosol, nucleoplasm, nucleus

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ABclonal:Western blot - DRG2 Rabbit pAb (A17476)}

Western blot - DRG2 Rabbit pAb (A17476)

Western blot analysis of various lysates using DRG2 Rabbit pAb (A17476) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Immunofluorescence - DRG2 Rabbit pAb (A17476)}

Immunofluorescence - DRG2 Rabbit pAb (A17476)

Immunofluorescence analysis of C6 cells using DRG2 Rabbit pAb (A17476) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - DRG2 Rabbit pAb (A17476)}

Immunofluorescence - DRG2 Rabbit pAb (A17476)

Immunofluorescence analysis of L929 cells using DRG2 Rabbit pAb (A17476) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.