제품 > 항체 > 다중클론항체(pAb)

DTH8 Rabbit pAb (A19184)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Oryza sativa

Western blot analysis of extracts of various tissues from the japonica rice (Oryza sativa L.) variety Zhonghua 11, using DTH8 Rabbit pAb (A19184) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 60s.

Overview

Product name	DTH8 Rabbit pAb
Catalog No.	A19184
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

Probable transcription factor involved in the regulation of flowering time under long day (LD) conditions. Functions as repressor of flowering, independently of HD1 and GHD7. Controls flowering time by negatively regulating the expression of EHD1 and HD3A. Regulates plant height by promoting cell elongation in the internodes. Component of the NF-Y/HAP transcription factor complex.

Immunogen information

Immunogen	Recombinant protein of Oryza sativa DTH8.
Sequence	Email for sequence
Gene ID
Swiss Prot
Synonyms	Hd5; DTH8; GHD8; HAP3H; NFYB11; OsHAP3H; OsNF-YB11
Calculated MW	30kDa
Observed MW	30kDa

Applications

Reactivity	Oryza sativa
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	young leaves (10 d), young roots (10 d)
Cellular location	cytoplasm, nucleus