제품 > 항체 > 다중클론항체(pAb)

DTYMK Rabbit pAb (A6370)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - DTYMK Rabbit pAb (A6370)

Western blot analysis of extracts of various cell lines, using DTYMK antibody (A6370) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunofluorescence - DTYMK Rabbit pAb (A6370)

Immunofluorescence analysis of L929 cells using DTYMK Rabbit pAb (A6370) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - DTYMK Rabbit pAb (A6370)

Immunofluorescence analysis of U-2 OS cells using DTYMK Rabbit pAb (A6370) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Overview

Product nameDTYMK Rabbit pAb
Catalog No.A6370
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Enables thymidylate kinase activity. Predicted to be involved in dTDP biosynthetic process; dTTP biosynthetic process; and dUDP biosynthetic process. Predicted to act upstream of or within cellular response to growth factor stimulus and nucleotide biosynthetic process. Predicted to be located in mitochondrial intermembrane space and mitochondrial matrix. Predicted to be active in cytosol; mitochondrion; and nucleus.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-212 of human DTYMK (NP_036277.2).
SequenceMAARRGALIVLEGVDRAGKSTQSRKLVEALCAAGHRAELLRFPERSTEIGKLLSSYLQKKSDVEDHSVHLLFSANRWEQVPLIKEKLSQGVTLVVDRYAFSGVAFTGAKENFSLDWCKQPDVGLPKPDLVLFLQLQLADAAKRGAFGHERYENGAFQERALRCFHQLMKDTTLNWKMVDASKSIEAVHEDIRVLSEDAIRTATEKPLGELWK
Gene ID
Swiss Prot
SynonymsCDC8; TMPK; TYMK; CONPM; PP3731
Calculated MW24kDa
Observed MW24kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samples22Rv1, HeLa, HepG2, Raji, MCF7, Mouse bone marrow, Mouse spleen, Rat testis
Cellular locationcytoplasm, cytosol, mitochondrion, nucleus

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

    ABclonal:Western blot - DTYMK Rabbit pAb (A6370)}

    Western blot - DTYMK Rabbit pAb (A6370)

    Western blot analysis of extracts of various cell lines, using DTYMK antibody (A6370) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 30s.
    ABclonal:Immunofluorescence - DTYMK Rabbit pAb (A6370)}

    Immunofluorescence - DTYMK Rabbit pAb (A6370)

    Immunofluorescence analysis of L929 cells using DTYMK Rabbit pAb (A6370) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - DTYMK Rabbit pAb (A6370)}

    Immunofluorescence - DTYMK Rabbit pAb (A6370)

    Immunofluorescence analysis of U-2 OS cells using DTYMK Rabbit pAb (A6370) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

    항체 (1)

    Secondary Antibodies (26)