제품 > 항체 > 다중클론항체(pAb)

DUSP15 Rabbit pAb (A16322)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - DUSP15 Rabbit pAb (A16322)

Western blot analysis of various lysates using DUSP15 Rabbit pAb (A16322) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunofluorescence - DUSP15 Rabbit pAb (A16322)

Immunofluorescence analysis of L929 cells using DUSP15 Rabbit pAb (A16322) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameDUSP15 Rabbit pAb
Catalog No.A16322
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene has both protein-tyrosine phophatase activity and serine/threonine-specific phosphatase activity, and therefore is known as a dual specificity phosphatase. This protein may function in the differentiation of oligodendrocytes. Alternative splicing results in multiple transcript variants.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 10-90 of human DUSP15 (NP_542178.2).
SequencePGLYLGNFIDAKDLDQLGRNKITHIISIHESPQPLLQDITYLRIPVADTPEVPIKKHFKECINFIHCCRLNGGNCLVHCFA
Gene ID
Swiss Prot
SynonymsVHY; C20orf57; DUSP15
Calculated MW32kDa
Observed MW32kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesPC-3, A-375, Mouse kidney, Rat kidney
Cellular locationcytosol, plasma membrane

Documents

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ABclonal:Western blot - DUSP15 Rabbit pAb (A16322)}

Western blot - DUSP15 Rabbit pAb (A16322)

Western blot analysis of various lysates using DUSP15 Rabbit pAb (A16322) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunofluorescence - DUSP15 Rabbit pAb (A16322)}

Immunofluorescence - DUSP15 Rabbit pAb (A16322)

Immunofluorescence analysis of L929 cells using DUSP15 Rabbit pAb (A16322) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.