제품 > 항체 > 다중클론항체(pAb)

DUSP22 Rabbit pAb (A7281)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - DUSP22 Rabbit pAb (A7281)

Western blot analysis of extracts of BT-474 cells, using DUSP22 antibody (A7281) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution._Lysates/proteins: 25μg per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 90s.

ABclonal:Immunofluorescence - DUSP22 Rabbit pAb (A7281)

Immunofluorescence analysis of A-549 cells using DUSP22 antibody (A7281). Blue: DAPI for nuclear staining.

Overview

Product nameDUSP22 Rabbit pAb
Catalog No.A7281
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Enables non-membrane spanning protein tyrosine phosphatase activity and protein tyrosine kinase binding activity. Involved in several processes, including cellular response to epidermal growth factor stimulus; negative regulation of focal adhesion assembly; and negative regulation of non-membrane spanning protein tyrosine kinase activity. Acts upstream of or within negative regulation of transcription by RNA polymerase II. Located in plasma membrane. Part of cytoplasm; filamentous actin; and leading edge of lamellipodium.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-184 of human DUSP22 (NP_064570.1).
SequenceMGNGMNKILPGLYIGNFKDARDAEQLSKNKVTHILSVHDSARPMLEGVKYLCIPAADSPSQNLTRHFKESIKFIHECRLRGESCLVHCLAGVSRSVTLVIAYIMTVTDFGWEDALHTVRAGRSCANPNVGFQRQLQEFEKHEVHQYRQWLKEEYGESPLQDAEEAKNILAAPGILKFWAFLRRL
Gene ID
Swiss Prot
SynonymsVHX; JKAP; JSP1; MKPX; JSP-1; MKP-x; LMWDSP2; LMW-DSP2
Calculated MW21kDa
Observed MW21kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesBT-474
Cellular locationCytoplasm, Nucleus

Documents

Certificate of Compliance

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    ABclonal:Western blot - DUSP22 Rabbit pAb (A7281)}

    Western blot - DUSP22 Rabbit pAb (A7281)

    Western blot analysis of extracts of BT-474 cells, using DUSP22 antibody (A7281) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution._Lysates/proteins: 25μg per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 90s.
    ABclonal:Immunofluorescence - DUSP22 Rabbit pAb (A7281)}

    Immunofluorescence - DUSP22 Rabbit pAb (A7281)

    Immunofluorescence analysis of A-549 cells using DUSP22 antibody (A7281). Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.