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DUSP6 Rabbit mAb (A0133)

Reviews (5) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - DUSP6 Rabbit mAb (A0133)

Western blot analysis of various lysates using DUSP6 Rabbit mAb (A0133) at 1:1000 dilution incubated at room temperature for 1.5 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 45s.

ABclonal:Western blot - DUSP6 Rabbit mAb (A0133)

Western blot analysis of various lysates using DUSP6 Rabbit mAb (A0133) at 1:1000 dilution incubated at room temperature for 1.5 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - DUSP6 Rabbit mAb (A0133)

Immunohistochemistry analysis of paraffin-embedded Human pancreas tissue using DUSP6 Rabbit mAb (A0133) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

Review
ABclonal: review for DUSP6 Rabbit mAb(A0133)
Review
ABclonal: review for DUSP6 Rabbit mAb(A0133)
Review
ABclonal: review for DUSP6 Rabbit mAb(A0133)
Review
ABclonal: review for DUSP6 Rabbit mAb(A0133)
Review
ABclonal: review for DUSP6 Rabbit mAb(A0133)

Overview

Product nameDUSP6 Rabbit mAb
Catalog No.A0133
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC0237
The protein encoded by this gene is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which are associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene product inactivates ERK2, is expressed in a variety of tissues with the highest levels in heart and pancreas, and unlike most other members of this family, is localized in the cytoplasm. Mutations in this gene have been associated with congenital hypogonadotropic hypogonadism. Alternatively spliced transcript variants have been found for this gene.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 282-381 of human DUSP6 (Q16828).
SequenceARGKNCGVLVHCLAGISRSVTVTVAYLMQKLNLSMNDAYDIVKMKKSNISPNFNFMGQLLDFERTLGLSSPCDNRVPAQQLYFTTPSNQNVYQVDSLQST
Gene ID
Swiss Prot
SynonymsHH19; MKP3; PYST1; DUSP6
Calculated MW42kDa
Observed MW40kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:2000
  • IHC-P 1:200 - 1:800
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesRat testis, Rat brain, BxPC-3, NIH/3T3, Mouse thymus
Cellular locationCytoplasm.
Customer validation

(HepG2 、PC-3 、COLO205, HepG2、PC-3、COLO205, HepG2、PC-3、COLO205 , hepg2 pc-3 oaw42 hbl-100 colo205 )

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - DUSP6 Rabbit mAb (A0133)}

Western blot - DUSP6 Rabbit mAb (A0133)

Western blot analysis of various lysates using DUSP6 Rabbit mAb (A0133) at 1:1000 dilution incubated at room temperature for 1.5 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 45s.
ABclonal:Western blot - DUSP6 Rabbit mAb (A0133)}

Western blot - DUSP6 Rabbit mAb (A0133)

Western blot analysis of various lysates using DUSP6 Rabbit mAb (A0133) at 1:1000 dilution incubated at room temperature for 1.5 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - DUSP6 Rabbit mAb (A0133)}

Immunohistochemistry - DUSP6 Rabbit mAb (A0133)

Immunohistochemistry analysis of paraffin-embedded Human pancreas tissue using DUSP6 Rabbit mAb (A0133) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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