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DUSP8 Rabbit pAb (A17990)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - DUSP8 Rabbit pAb (A17990)

Western blot analysis of lysates from Rat lung, using DUSP8 Rabbit pAb (A17990) at 1:2000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Immunofluorescence - DUSP8 Rabbit pAb (A17990)

Immunofluorescence analysis of HeLa cells using DUSP8 Rabbit pAb (A17990) at dilution of 1:1000 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - DUSP8 Rabbit pAb (A17990)

Immunofluorescence analysis of MCF7 cells using DUSP8 Rabbit pAb (A17990) at dilution of 1:1000 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - DUSP8 Rabbit pAb (A17990)

Immunofluorescence analysis of NIH/3T3 cells using DUSP8 Rabbit pAb (A17990) at dilution of 1:1000 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - DUSP8 Rabbit pAb (A17990)

Immunofluorescence analysis of PC-12 cells using DUSP8 Rabbit pAb (A17990) at dilution of 1:1000 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameDUSP8 Rabbit pAb
Catalog No.A17990
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which is associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene product inactivates SAPK/JNK and p38, is expressed predominantly in the adult brain, heart, and skeletal muscle, is localized in the cytoplasm, and is induced by nerve growth factor and insulin. An intronless pseudogene for DUSP8 is present on chromosome 10q11.2.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human DUSP8 (NP_004411.2).
SequenceMAGDRLPRKVMDAKKLASLLRGGPGGPLVIDSRSFVEYNSWHVLSSVNICCSKLVKRRLQQGKVTIAELIQPAARSQVEATEPQDVVVYDQSTRDASVLA
Gene ID
Swiss Prot
SynonymsHB5; HVH8; HVH-5; C11orf81; DUSP8
Calculated MW66kDa
Observed MW66kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:5000
  • IF/ICC 1:500 - 1:1000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesRat lung
Cellular locationCytoplasm, Nucleus.
Customer validation

RT-PCR(Mus musculus)

WB(Mus musculus)

Documents

Certificate of Compliance

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    ABclonal:Western blot - DUSP8 Rabbit pAb (A17990)}

    Western blot - DUSP8 Rabbit pAb (A17990)

    Western blot analysis of lysates from Rat lung, using DUSP8 Rabbit pAb (A17990) at 1:2000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 60s.
    ABclonal:Immunofluorescence - DUSP8 Rabbit pAb (A17990)}

    Immunofluorescence - DUSP8 Rabbit pAb (A17990)

    Immunofluorescence analysis of HeLa cells using DUSP8 Rabbit pAb (A17990) at dilution of 1:1000 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - DUSP8 Rabbit pAb (A17990)}

    Immunofluorescence - DUSP8 Rabbit pAb (A17990)

    Immunofluorescence analysis of MCF7 cells using DUSP8 Rabbit pAb (A17990) at dilution of 1:1000 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - DUSP8 Rabbit pAb (A17990)}

    Immunofluorescence - DUSP8 Rabbit pAb (A17990)

    Immunofluorescence analysis of NIH/3T3 cells using DUSP8 Rabbit pAb (A17990) at dilution of 1:1000 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - DUSP8 Rabbit pAb (A17990)}

    Immunofluorescence - DUSP8 Rabbit pAb (A17990)

    Immunofluorescence analysis of PC-12 cells using DUSP8 Rabbit pAb (A17990) at dilution of 1:1000 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A17990? Please let us know so that we can cite the reference in this datasheet.