제품 > 항체 > 다중클론항체(pAb)

DVL1 Rabbit pAb (A10536)

Reviews (2)Publications (3) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - DVL1 Rabbit pAb (A10536)

Western blot analysis of various lysates, using DVL1 Rabbit pAb (A10536) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.

ABclonal:Immunohistochemistry - DVL1 Rabbit pAb (A10536)

Immunohistochemistry analysis of paraffin-embedded Rat testis using DVL1 Rabbit pAb (A10536) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunohistochemistry - DVL1 Rabbit pAb (A10536)

Immunohistochemistry analysis of paraffin-embedded Mouse testis using DVL1 Rabbit pAb (A10536) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunofluorescence - DVL1 Rabbit pAb (A10536)

Immunofluorescence analysis of NIH-3T3 cells using DVL1 Rabbit pAb (A10536) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - DVL1 Rabbit pAb (A10536)

Immunofluorescence analysis of C6 cells using DVL1 Rabbit pAb (A10536) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Review
ABclonal: review for DVL1 Rabbit pAb(A10536)
Review
ABclonal: review for DVL1 Rabbit pAb(A10536)

Overview

Product nameDVL1 Rabbit pAb
Catalog No.A10536
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
DVL1, the human homolog of the Drosophila dishevelled gene (dsh) encodes a cytoplasmic phosphoprotein that regulates cell proliferation, acting as a transducer molecule for developmental processes, including segmentation and neuroblast specification. DVL1 is a candidate gene for neuroblastomatous transformation. The Schwartz-Jampel syndrome and Charcot-Marie-Tooth disease type 2A have been mapped to the same region as DVL1. The phenotypes of these diseases may be consistent with defects which might be expected from aberrant expression of a DVL gene during development.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 510-640 of human DVL1 (NP_004412.2).
SequenceGQGYPYQYPGPPPCFPPAYQDPGFSYGSGSTGSQQSEGSKSSGSTRSSRRAPGREKERRAAGAGGSGSESDHTAPSGVGSSWRERPAGQLSRGSSPRSQASATAPGLPPPHPTTKAYTVVGGPPGGPPVRE
Gene ID
Swiss Prot
SynonymsDVL; DRS2; DVL1L1; DVL1P1; DVL1
Calculated MW75kDa
Observed MW75kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHeLa, PC-3, 293T
Cellular locationCell membrane, Cytoplasm, Cytoplasmic side, Cytoplasmic vesicle, Peripheral membrane protein, cytosol.
Customer validation

(293T)

WB(Capra hircus, Rattus norvegicus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

ABclonal:Western blot - DVL1 Rabbit pAb (A10536)}

Western blot - DVL1 Rabbit pAb (A10536)

Western blot analysis of various lysates, using DVL1 Rabbit pAb (A10536) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.
ABclonal:Immunohistochemistry - DVL1 Rabbit pAb (A10536)}

Immunohistochemistry - DVL1 Rabbit pAb (A10536)

Immunohistochemistry analysis of paraffin-embedded Rat testis using DVL1 Rabbit pAb (A10536) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
ABclonal:Immunohistochemistry - DVL1 Rabbit pAb (A10536)}

Immunohistochemistry - DVL1 Rabbit pAb (A10536)

Immunohistochemistry analysis of paraffin-embedded Mouse testis using DVL1 Rabbit pAb (A10536) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
ABclonal:Immunofluorescence - DVL1 Rabbit pAb (A10536)}

Immunofluorescence - DVL1 Rabbit pAb (A10536)

Immunofluorescence analysis of NIH-3T3 cells using DVL1 Rabbit pAb (A10536) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - DVL1 Rabbit pAb (A10536)}

Immunofluorescence - DVL1 Rabbit pAb (A10536)

Immunofluorescence analysis of C6 cells using DVL1 Rabbit pAb (A10536) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Submit an review for A10536

Please submit reviews to your technical sales specialist directly. Alternatively, email us at info@abclonal.co.kr

Reviews List

Publishing research using A10536? Please let us know so that we can cite the reference in this datasheet.

항체 (1)

Secondary Antibodies (26)