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DYNLL2 Rabbit pAb (A13888)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - DYNLL2 Rabbit pAb (A13888)

Western blot analysis of various lysates using DYNLL2 Rabbit pAb (A13888) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates / proteins: 25 μg per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Western blot - DYNLL2 Rabbit pAb (A13888)

Western blot analysis of various lysates using DYNLL2 Rabbit pAb (A13888) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates / proteins: 25 μg per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Immunofluorescence - DYNLL2 Rabbit pAb (A13888)

Immunofluorescence analysis of NIH/3T3 cells using DYNLL2 Rabbit pAb (A13888) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameDYNLL2 Rabbit pAb
Catalog No.A13888
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Predicted to enable dynein intermediate chain binding activity and dynein light intermediate chain binding activity. Predicted to be involved in cilium assembly. Located in 9+0 non-motile cilium and centrosome. Is active in glutamatergic synapse and postsynapse.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-89 of human DYNLL2 (NP_542408.1).
SequenceMSDRKAVIKNADMSEDMQQDAVDCATQAMEKYNIEKDIAAYIKKEFDKKYNPTWHCIVGRNFGSYVTHETKHFIYFYLGQVAILLFKSG
Gene ID
Swiss Prot
SynonymsDlc2; DNCL1B; RSPH22; DYNLL2
Calculated MW10kDa
Observed MW12kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesMouse testis, Mouse brain
Cellular locationCytoplasm, cytoskeleton
Customer validation

WB(Capra hircus)

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - DYNLL2 Rabbit pAb (A13888)}

Western blot - DYNLL2 Rabbit pAb (A13888)

Western blot analysis of various lysates using DYNLL2 Rabbit pAb (A13888) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates / proteins: 25 μg per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Western blot - DYNLL2 Rabbit pAb (A13888)}

Western blot - DYNLL2 Rabbit pAb (A13888)

Western blot analysis of various lysates using DYNLL2 Rabbit pAb (A13888) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates / proteins: 25 μg per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Immunofluorescence - DYNLL2 Rabbit pAb (A13888)}

Immunofluorescence - DYNLL2 Rabbit pAb (A13888)

Immunofluorescence analysis of NIH/3T3 cells using DYNLL2 Rabbit pAb (A13888) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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항체 (1)

Secondary Antibodies (25)