DiMethyl-DNMT3A-K44 Rabbit pAb (A16012)

Datasheet

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

ABclonal:Immunofluorescence - DiMethyl-DNMT3A-K44 Rabbit pAb (A16012)

Immunofluorescence analysis of U2OS cells using DiMethyl-DNMT3A-K44 antibody (A16012) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of C6 cells using DiMethyl-DNMT3A-K44 antibody (A16012) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of HeLa cells using DiMethyl-DNMT3A-K44 antibody (A16012) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH/3T3 cells using DiMethyl-DNMT3A-K44 antibody (A16012) at dilution of 1:100. Blue: DAPI for nuclear staining.

Overview

Product name	DiMethyl-DNMT3A-K44 Rabbit pAb
Catalog No.	A16012
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

This is one of two related genes encoding de novo DNA methyltransferases, which are responsible for the establishment of DNA methylation patterns in embryos. Loss of function of this gene causes developmental defects in multiple different organ systems. There is a pseudogene for this gene located on chromosome 3. Alternatively spliced transcript variants encoding multiple isoforms have been observed.

Immunogen information

Immunogen	A synthetic dimethylated peptide around K44 of mouse DNMT3A (NP_031898.1).
Sequence	ARKVG
Gene ID
Swiss Prot
Synonyms	MmuIIIA
Calculated MW	102kDa
Observed MW

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	IF/ICC 1:50 - 1:200
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key application	Immunofluorescence
Positive samples
Cellular location	Cytoplasm, Nucleus