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Dysferlin Rabbit mAb (A19572)

Publications (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - Dysferlin Rabbit mAb (A19572)

Western blot analysis of lysates from RD cells, using Dysferlin (Romeo) Rabbit mAb (A19572) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - Dysferlin Rabbit mAb (A19572)

Immunohistochemistry analysis of paraffin-embedded Human placenta using Dysferlin (Romeo) Rabbit mAb (A19572) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

Overview

Product nameDysferlin Rabbit mAb
Catalog No.A19572
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC2178
The protein encoded by this gene belongs to the ferlin family and is a skeletal muscle protein found associated with the sarcolemma. It is involved in muscle contraction and contains C2 domains that play a role in calcium-mediated membrane fusion events, suggesting that it may be involved in membrane regeneration and repair. In addition, the protein encoded by this gene binds caveolin-3, a skeletal muscle membrane protein which is important in the formation of caveolae. Specific mutations in this gene have been shown to cause autosomal recessive limb girdle muscular dystrophy type 2B (LGMD2B) as well as Miyoshi myopathy. Alternative splicing results in multiple transcript variants.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 50-150 of human Dysferlin (Romeo) (O75923).
SequenceFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDV
Gene ID
Swiss Prot
SynonymsMMD1; FER1L1; LGMD2B; LGMDR2; Dysferlin (Romeo)
Calculated MW237kDa
Observed MW280kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesRD
Cellular locationCytoplasmic vesicle, Endosome, Extracellular exosome, Plasma membrane, T-tubule
Customer validation

WB(Homo sapiens, Rattus norvegicus)

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - Dysferlin Rabbit mAb (A19572)}

    Western blot - Dysferlin Rabbit mAb (A19572)

    Western blot analysis of lysates from RD cells, using Dysferlin (Romeo) Rabbit mAb (A19572) at 1:1000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 30s.
    ABclonal:Immunohistochemistry - Dysferlin Rabbit mAb (A19572)}

    Immunohistochemistry - Dysferlin Rabbit mAb (A19572)

    Immunohistochemistry analysis of paraffin-embedded Human placenta using Dysferlin (Romeo) Rabbit mAb (A19572) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A19572? Please let us know so that we can cite the reference in this datasheet.

    Secondary Antibodies (26)