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E2F1 Rabbit mAb (A19579)

Review (1)Publications (7) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - E2F1 Rabbit mAb (A19579)

Western blot analysis of various lysates using E2F1 Rabbit mAb (A19579) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Western blot - E2F1 Rabbit mAb (A19579)

Western blot analysis of lysates from rat spleen, using E2F1 Rabbit mAb (A19579) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1min.

ABclonal:Immunoprecipitation - E2F1 Rabbit mAb (A19579)

Immunoprecipitation analysis of 300 μg extracts of HT-29 cells using 3 μg E2F1 antibody (A19579). Western blot was performed from the immunoprecipitate using E2F1 antibody (A19579) at a dilution of 1:1000.

Review
ABclonal: review for E2F1 Rabbit mAb(A19579)

Overview

Product nameE2F1 Rabbit mAb
Catalog No.A19579
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC0058
The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 338-437 of human E2F1 (Q01094).
SequencePPSSLTTDPSQSLLSLEQEPLLSRMGSLRAPVDEDRLSPLVAADSLLEHVREDFSGLLPEEFISLSPPHEALDYHFGLEEGEGIRDLFDCDFGDLTPLDF
Gene ID
Swiss Prot
SynonymsRBP3; E2F-1; RBAP1; RBBP3; E2F1
Calculated MW47kDa
Observed MW70kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunoprecipitation    
Positive samplesU-87MG, HT-29, HeLa, A-431, Mouse testis, Mouse brain, Rat thymus, Rat spleen
Cellular locationNucleus
Customer validation

(Hela)

WB(Homo sapiens, Mus musculus)

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - E2F1 Rabbit mAb (A19579)}

Western blot - E2F1 Rabbit mAb (A19579)

Western blot analysis of various lysates using E2F1 Rabbit mAb (A19579) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Western blot - E2F1 Rabbit mAb (A19579)}

Western blot - E2F1 Rabbit mAb (A19579)

Western blot analysis of lysates from rat spleen, using E2F1 Rabbit mAb (A19579) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1min.
ABclonal:Immunoprecipitation - E2F1 Rabbit mAb (A19579)}

Immunoprecipitation - E2F1 Rabbit mAb (A19579)

Immunoprecipitation analysis of 300 μg extracts of HT-29 cells using 3 μg E2F1 antibody (A19579). Western blot was performed from the immunoprecipitate using E2F1 antibody (A19579) at a dilution of 1:1000.

* For research use only. Not for therapeutic or diagnostic purposes.

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항체 (2)

Secondary Antibodies (26)