제품 > 항체 > 다중클론항체(pAb)

E2F6 Rabbit pAb (A2718)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse

Western blot analysis of lysates from Mouse lung using E2F6 Rabbit pAb (A2718) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunofluorescence - E2F6 Rabbit pAb (A2718)

Immunofluorescence analysis of U2OS cells using E2F6 Rabbit pAb (A2718). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - E2F6 Rabbit pAb (A2718)

Immunoprecipitation analysis of 200 μg extracts of MCF-7 cells using 3 μg E2F6 antibody (A2718). Western blot was performed from the immunoprecipitate using E2F6 antibody (A2718) at a dilution of 1:500.

ABclonal:Chromatin Immunoprecipitation - E2F6 Rabbit pAb (A2718)

Chromatin immunoprecipitation analysis of extracts of 293T cells, using E2F6 antibody (A2718) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Overview

Product name	E2F6 Rabbit pAb
Catalog No.	A2718
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene encodes a member of a family of transcription factors that play a crucial role in the control of the cell cycle. The protein encoded by this gene lacks the transactivation and tumor suppressor protein association domains found in other family members, and contains a modular suppression domain that functions in the inhibition of transcription. It interacts in a complex with chromatin modifying factors. There are pseudogenes for this gene on chromosomes 22 and X. Alternative splicing results in multiple transcript variants.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 1-281 of human E2F6 (NP_937987.2).
Sequence	MSQQRPARKLPSLLLDPTEETVRRRCRDPINVEGLLPSKIRINLEDNVQYVSMRKALKVKRPRFDVSLVYLTRKFMDLVRSAPGGILDLNKVATKLGVRKRRVYDITNVLDGIDLVEKKSKNHIRWIGSDLSNFGAVPQQKKLQEELSDLSAMEDALDELIKDCAQQLFELTDDKENERLAYVTYQDIHSIQAFHEQIVIAVKAPAETRLDVPAPREDSITVHIRSTNGPIDVYLCEVEQGQTSNKRSEGVGTSSSESTHPEGPEEEENPQQSEELLEVSN
Gene ID
Swiss Prot
Synonyms	E2F-6; E2F6
Calculated MW	32kDa
Observed MW	35kDa

Applications

Reactivity	Human, Mouse
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000 IF/ICC 1:50 - 1:200 IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements. ChIP 5μg antibody for 10μg-15μg of Chromatin
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting Immunofluorescence Immunoprecipitation
Positive samples	Mouse lung
Cellular location	Nucleus.
Customer validation	WB(Homo sapiens)

Documents

Certificate of Compliance

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Research Area

E2F6 Rabbit pAb images

Western blot - E2F6 Rabbit pAb (A2718)

Immunofluorescence - E2F6 Rabbit pAb (A2718)

Immunofluorescence analysis of U2OS cells using E2F6 Rabbit pAb (A2718). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunoprecipitation - E2F6 Rabbit pAb (A2718)

Chromatin Immunoprecipitation - E2F6 Rabbit pAb (A2718)

* For research use only. Not for therapeutic or diagnostic purposes.