제품 > 항체 > 다중클론항체(pAb)

E2F7 Rabbit pAb (A15211)

Publications (5) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - E2F7 Rabbit pAb (A15211)

Western blot analysis of various lysates using E2F7 Rabbit pAb (A15211) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Western blot - E2F7 Rabbit pAb (A15211)

Western blot analysis of various lysates using E2F7 Rabbit pAb (A15211) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 60s.

Overview

Product nameE2F7 Rabbit pAb
Catalog No.A15211
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Enables DNA-binding transcription factor activity; cis-regulatory region sequence-specific DNA binding activity; and identical protein binding activity. Involved in several processes, including DNA damage response, signal transduction by p53 class mediator; regulation of transcription, DNA-templated; and sprouting angiogenesis. Located in nuclear speck.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 540-740 of human E2F7 (NP_976328.2).
SequenceLLAGQPLVYVPSASLFMLYGSLQEGPASGSGSERDDRSSEAPATVELSSAPSAQKRLCEERKPQEEDEPATKRQSREYEDGPLSLVMPKKPSDSTDLASPKTMGNRASIPLKDIHVNGQLPAAEEISGKATANSLVSSEWGNPSRNTDVEKPSKENESTKEPSLLQYLCVQSPAGLNGFNVLLSGSQTPPTVGPSSGQLPS
Gene ID
Swiss Prot
SynonymsE2F7
Calculated MW100kDa
Observed MW105kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesHeLa, SP2/0, Mouse spleen, Rat spleen
Cellular locationNucleus
Customer validation

WB(Homo sapiens)

IHC(Homo sapiens,Mus musculus)

WB(Homo sapiens,Mus musculus)

ABclonal:Western blot - E2F7 Rabbit pAb (A15211)}

Western blot - E2F7 Rabbit pAb (A15211)

Western blot analysis of various lysates using E2F7 Rabbit pAb (A15211) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Western blot - E2F7 Rabbit pAb (A15211)}

Western blot - E2F7 Rabbit pAb (A15211)

Western blot analysis of various lysates using E2F7 Rabbit pAb (A15211) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 60s.

* For research use only. Not for therapeutic or diagnostic purposes.

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