제품 > 항체 > 다중클론항체(pAb)

EAF2 Rabbit pAb (A7119)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - EAF2 Rabbit pAb (A7119)

Western blot analysis of extracts of various cell lines, using EAF2 antibody (A7119) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.

ABclonal:Immunofluorescence - EAF2 Rabbit pAb (A7119)

Immunofluorescence analysis of A-549 cells using EAF2 antibody (A7119).

Overview

Product nameEAF2 Rabbit pAb
Catalog No.A7119
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Enables transcription elongation regulator activity. Involved in positive regulation of transcription by RNA polymerase II and regulation of transcription elongation from RNA polymerase II promoter. Part of transcription elongation factor complex. Biomarker of prostate cancer.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-170 of human EAF2 (NP_060926.2).
SequenceMNSAAGFSHLDRRERVLKLGESFEKQPRCAFHTVRYDFKPASIDTSSEGYLEVGEGEQVTITLPNIEGSTPPVTVFKGSKKPYLKECILIINHDTGECRLEKLSSNITVKKTRVEGSSKIQYRKEQQQQQMWNSARTPNLVKHSPSEDKMSPASPIDDIERELKAEASLM
Gene ID
Swiss Prot
SynonymsU19; BM040; TRAITS
Calculated MW29kDa
Observed MW35kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesHepG2, Mouse kidney, Mouse spleen, Rat kidney
Cellular locationNucleus speckle

Documents

Certificate of Compliance

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    ABclonal:Western blot - EAF2 Rabbit pAb (A7119)}

    Western blot - EAF2 Rabbit pAb (A7119)

    Western blot analysis of extracts of various cell lines, using EAF2 antibody (A7119) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Enhanced Kit (RM00021).
    Exposure time: 90s.
    ABclonal:Immunofluorescence - EAF2 Rabbit pAb (A7119)}

    Immunofluorescence - EAF2 Rabbit pAb (A7119)

    Immunofluorescence analysis of A-549 cells using EAF2 antibody (A7119).

    * For research use only. Not for therapeutic or diagnostic purposes.