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ECSIT Rabbit pAb (A7804)

Publications (4) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - ECSIT Rabbit pAb (A7804)

Western blot analysis of extracts of HepG2 cells, using ECSIT antibody (A7804) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunofluorescence - ECSIT Rabbit pAb (A7804)

Immunofluorescence analysis of H9C2 cells using ECSIT Rabbit pAb (A7804) at dilution of 100 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - ECSIT Rabbit pAb (A7804)

Immunofluorescence analysis of L929 cells using ECSIT Rabbit pAb (A7804) at dilution of 100 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - ECSIT Rabbit pAb (A7804)

Immunofluorescence analysis of U2OS cells using ECSIT Rabbit pAb (A7804) at dilution of 100 (40x lens). Blue: DAPI for nuclear staining.

Overview

Product nameECSIT Rabbit pAb
Catalog No.A7804
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Predicted to enable DNA-binding transcription factor activity and chromatin binding activity. Involved in regulation of oxidoreductase activity and regulation of protein complex stability. Located in cytosol; mitochondrion; and nucleoplasm.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 50-296 of human ECSIT (NP_001135936.1).
SequenceSEQSLVPSPPEPRQRPTKALVPFEDLFGQAPGGERDKASFLQTVQKFAEHSVRKRGHIDFIYLALRKMREYGVERDLAVYNQLLNIFPKEVFRPRNIIQRIFVHYPRQQECGIAVLEQMENHGVMPNKETEFLLIQIFGRKSYPMLKLVRLKLWFPRFMNVNPFPVPRDLPQDPVELAMFGLRHMEPDLSARVTIYQVPLPKDSTGAADPPQPHIVGSGRDAGGVEPLLPDAAGPGVCEEWLGQLRV
Gene ID
Swiss Prot
SynonymsSITPEC
Calculated MW49kDa
Observed MW49kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesHepG2
Cellular locationCytoplasm, Mitochondrion, Nucleus
Customer validation

WB(Mus musculus, Rattus norvegicus, Homo sapiens)

IHC(Mus musculus)

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - ECSIT Rabbit pAb (A7804)}

Western blot - ECSIT Rabbit pAb (A7804)

Western blot analysis of extracts of HepG2 cells, using ECSIT antibody (A7804) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunofluorescence - ECSIT Rabbit pAb (A7804)}

Immunofluorescence - ECSIT Rabbit pAb (A7804)

Immunofluorescence analysis of H9C2 cells using ECSIT Rabbit pAb (A7804) at dilution of 100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - ECSIT Rabbit pAb (A7804)}

Immunofluorescence - ECSIT Rabbit pAb (A7804)

Immunofluorescence analysis of L929 cells using ECSIT Rabbit pAb (A7804) at dilution of 100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - ECSIT Rabbit pAb (A7804)}

Immunofluorescence - ECSIT Rabbit pAb (A7804)

Immunofluorescence analysis of U2OS cells using ECSIT Rabbit pAb (A7804) at dilution of 100 (40x lens). Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A7804? Please let us know so that we can cite the reference in this datasheet.

Secondary Antibodies (26)