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EFHD2 Rabbit pAb (A14926)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Mouse

ABclonal:Western blot - EFHD2 Rabbit pAb (A14926)

Western blot analysis of lysates from mouse lung, using EFHD2 Rabbit pAb (A14926) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

Overview

Product nameEFHD2 Rabbit pAb
Catalog No.A14926
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Enables cadherin binding activity. Predicted to be located in plasma membrane.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-78 of human EFHD2 (NP_077305.2).
SequenceMATDELATKLSRRLQMEGEGGGETPEQPGLNGAAAAAAGAPDEAAEALGSADCELSAKLLRRADLNQGIGEPQSPSRR
Gene ID
Swiss Prot
SynonymsSWS1; EFHD2
Calculated MW27kDa
Observed MW27kDa
ReactivityMouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesMouse lung
Cellular locationMembrane raft
Customer validation

WB(Mus musculus)

Documents

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ABclonal:Western blot - EFHD2 Rabbit pAb (A14926)}

Western blot - EFHD2 Rabbit pAb (A14926)

Western blot analysis of lysates from mouse lung, using EFHD2 Rabbit pAb (A14926) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A14926? Please let us know so that we can cite the reference in this datasheet.

ELISA 키트 (1)

Secondary Antibodies (26)