제품 > 항체 > 다중클론항체(pAb)

EHD4 Rabbit pAb (A13766)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - EHD4 Rabbit pAb (A13766)

Western blot analysis of various lysates using EHD4 Rabbit pAb (A13766) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

Overview

Product nameEHD4 Rabbit pAb
Catalog No.A13766
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Enables cadherin binding activity. Involved in endocytic recycling and protein homooligomerization. Located in endoplasmic reticulum and recycling endosome membrane.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 362-541 of human EHD4 (NP_644670.1).
SequenceMQEQLENYDFTKFHSLKPKLIEAVDNMLSNKISPLMNLISQEETSTPTQLVQGGAFDGTTEGPFNQGYGEGAKEGADEEEWVVAKDKPVYDELFYTLSPINGKISGVNAKKEMVTSKLPNSVLGKIWKLADCDCDGMLDEEEFALAKHLIKIKLDGYELPSSLPPHLVPPSHRKSLPKAD
Gene ID
Swiss Prot
SynonymsPAST4; EHD4
Calculated MW61kDa
Observed MW72kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesHeLa, 293T, Mouse brain, Mouse heart
Cellular locationCell membrane, Cytoplasmic side, Early endosome membrane, Peripheral membrane protein, Recycling endosome membrane

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ABclonal:Western blot - EHD4 Rabbit pAb (A13766)}

Western blot - EHD4 Rabbit pAb (A13766)

Western blot analysis of various lysates using EHD4 Rabbit pAb (A13766) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

* For research use only. Not for therapeutic or diagnostic purposes.

Secondary Antibodies (26)