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EIF2S3 Rabbit pAb (A6581)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - EIF2S3 Rabbit pAb (A6581)

Western blot analysis of various lysates using EIF2S3 Rabbit pAb (A6581) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - EIF2S3 Rabbit pAb (A6581)

Immunohistochemistry analysis of paraffin-embedded Human liver using EIF2S3 Rabbit pAb (A6581) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunohistochemistry - EIF2S3 Rabbit pAb (A6581)

Immunohistochemistry analysis of paraffin-embedded Human stomach using EIF2S3 Rabbit pAb (A6581) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunohistochemistry - EIF2S3 Rabbit pAb (A6581)

Immunohistochemistry analysis of paraffin-embedded Rat brain using EIF2S3 Rabbit pAb (A6581) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

Overview

Product nameEIF2S3 Rabbit pAb
Catalog No.A6581
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is the largest subunit of a heterotrimeric GTP-binding protein involved in the recruitment of methionyl-tRNA(i) to the 40 S ribosomal subunit.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human EIF2S3 (NP_001406.1).
SequenceMAGGEAGVTLGQPHLSRQDLTTLDVTKLTPLSHEVISRQATINIGTIGHVAHGKSTVVKAISGVHTVRFKNELERNITIKLGYANAKIYKLDDPSCPRPECYRSCGSSTPDEFPTDIPGTKGNFKLVRHVSFVDCPGHDILMATMLNGAAVMDAALLLIAGNESCPQPQTSEHLAAIEIMKLKHILILQNKIDLVKESQAKEQYEQILAFVQGTVAEGAPIIPISAQLKYNIEVVCEYIVKKIPVPPRDF
Gene ID
Swiss Prot
SynonymsEIF2; EIF2G; MEHMO; MRXSBRK; eIF-2gA; EIF2gamma; EIF2S3
Calculated MW51kDa
Observed MW51kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesHeLa, HepG2, Raji, U-251MG, Mouse liver, Mouse thymus, Rat liver
Cellular locationcytoplasm, cytosol, eukaryotic translation initiation factor 2 complex, extracellular exosome.

Documents

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ABclonal:Western blot - EIF2S3 Rabbit pAb (A6581)}

Western blot - EIF2S3 Rabbit pAb (A6581)

Western blot analysis of various lysates using EIF2S3 Rabbit pAb (A6581) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunohistochemistry - EIF2S3 Rabbit pAb (A6581)}

Immunohistochemistry - EIF2S3 Rabbit pAb (A6581)

Immunohistochemistry analysis of paraffin-embedded Human liver using EIF2S3 Rabbit pAb (A6581) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
ABclonal:Immunohistochemistry - EIF2S3 Rabbit pAb (A6581)}

Immunohistochemistry - EIF2S3 Rabbit pAb (A6581)

Immunohistochemistry analysis of paraffin-embedded Human stomach using EIF2S3 Rabbit pAb (A6581) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
ABclonal:Immunohistochemistry - EIF2S3 Rabbit pAb (A6581)}

Immunohistochemistry - EIF2S3 Rabbit pAb (A6581)

Immunohistochemistry analysis of paraffin-embedded Rat brain using EIF2S3 Rabbit pAb (A6581) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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Secondary Antibodies (26)