Reviews (2)Publications (3) Datasheet
Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse
Western blot analysis of RAW 264.7, using EIF3A Rabbit pAb (A0573) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
Immunofluorescence analysis of U2OS cells using EIF3A antibody (A0573) at dilution of 1:100. Blue: DAPI for nuclear staining.
| Product name | EIF3A Rabbit pAb |
|---|---|
| Catalog No. | A0573 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human EIF3A (NP_003741.1). |
|---|---|
| Sequence | MPAYFQRPENALKRANEFLEVGKKQPALDVLYDVMKSKKHRTWQKIHEPIMLKYLELCVDLRKSHLAKEGLYQYKNICQQVNIKSLEDVVRAYLKMAEEKTEAAKEESQQMVLDIEDLDNIQTPESVLLSAVSGEDTQDRTDRLLLTPWVKFLWESYRQCLDLLRNNSRVERLYHDIAQQAFKFCLQYTRKAEFRKLCDNLRMHLSQIQRHHNQSTAINLNNPESQSMHLETRLVQLDSAISMELWQEAF |
| Gene ID | |
| Swiss Prot | |
| Synonyms | EIF3; P167; p180; p185; TIF32; EIF3S10; eIF3-p170; eIF3-theta |
| Calculated MW | 167kDa |
| Observed MW | 166kDa |
| Reactivity | Human, Mouse |
|---|---|
| Tested applications | WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition |
| Recommended dilution |
|
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Key application | Western blotting Immunofluorescence |
| Positive samples | RAW 264.7 |
| Cellular location | Cytoplasm |
| Customer validation | (RD cell, HeLa) WB(Rana amurensis Boulenger, Homo sapiens, Mus musculus) IF(Homo sapiens) |
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_WB_01.jpg?t=1696639546 "ABclonal:Western blot - EIF3A Rabbit pAb (A0573)")
