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ELAVL3 Rabbit pAb (A6091)

Publications (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - ELAVL3 Rabbit pAb (A6091)

Western blot analysis of various lysates, using ELAVL3 Rabbit pAb (A6091) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 0.8s.

ABclonal:Immunohistochemistry - ELAVL3 Rabbit pAb (A6091)

Immunohistochemistry analysis of paraffin-embedded Rat brain using ELAVL3 Rabbit pAb (A6091) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunohistochemistry - ELAVL3 Rabbit pAb (A6091)

Immunohistochemistry analysis of paraffin-embedded Mouse brain using ELAVL3 Rabbit pAb (A6091) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunofluorescence - ELAVL3 Rabbit pAb (A6091)

Immunofluorescence analysis of paraffin-embedded mouse brain using ELAVL3 Rabbit pAb (A6091) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameELAVL3 Rabbit pAb
Catalog No.A6091
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
A member of the ELAVL protein family, ELAV-like 3 is a neural-specific RNA-binding protein which contains three RNP-type RNA recognition motifs. The observation that ELAVL3 is one of several Hu antigens (neuronal-specific RNA-binding proteins) recognized by the anti-Hu serum antibody present in sera from patients with paraneoplastic encephalomyelitis and sensory neuronopathy (PEM/PSN) suggests it has a role in neurogenesis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 161-260 of human ELAVL3 (NP_001411.2).
SequenceVTGVSRGVGFIRFDKRIEAEEAIKGLNGQKPLGAAEPITVKFANNPSQKTGQALLTHLYQSSARRYAGPLHHQTQRFRLDNLLNMAYGVKSPLSLIARFSPIAIDGMSGLAGVGLSGGAAGAGWCIFVYNLSPEADESVLWQLFGPFGAVTNVKVIRDFTTNKCKGFGFVTMTNYDEAAMAIASLNGYRLGERVLQVSFKTSKQHKA
Gene ID
Swiss Prot
SynonymsHUC; HUCL; PLE21; ELAVL3
Calculated MW40kDa
Observed MW37kDaa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesSH-SY5Y, Mouse brain, Mouse spinal cord, Rat brain
Cellular location
Customer validation

WB(Homo sapiens)

IP(Homo sapiens)

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - ELAVL3 Rabbit pAb (A6091)}

    Western blot - ELAVL3 Rabbit pAb (A6091)

    Western blot analysis of various lysates, using ELAVL3 Rabbit pAb (A6091) at 1:500 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 0.8s.
    ABclonal:Immunohistochemistry - ELAVL3 Rabbit pAb (A6091)}

    Immunohistochemistry - ELAVL3 Rabbit pAb (A6091)

    Immunohistochemistry analysis of paraffin-embedded Rat brain using ELAVL3 Rabbit pAb (A6091) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
    ABclonal:Immunohistochemistry - ELAVL3 Rabbit pAb (A6091)}

    Immunohistochemistry - ELAVL3 Rabbit pAb (A6091)

    Immunohistochemistry analysis of paraffin-embedded Mouse brain using ELAVL3 Rabbit pAb (A6091) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
    ABclonal:Immunofluorescence - ELAVL3 Rabbit pAb (A6091)}

    Immunofluorescence - ELAVL3 Rabbit pAb (A6091)

    Immunofluorescence analysis of paraffin-embedded mouse brain using ELAVL3 Rabbit pAb (A6091) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A6091? Please let us know so that we can cite the reference in this datasheet.

    Secondary Antibodies (26)