제품 > 항체 > 다중클론항체(pAb)

ELSPBP1 Rabbit pAb (A15882)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Mouse, Rat

ABclonal:Western blot - ELSPBP1 Rabbit pAb (A15882)

Western blot analysis of various lysates using ELSPBP1 Rabbit pAb (A15882) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunofluorescence - ELSPBP1 Rabbit pAb (A15882)

Immunofluorescence analysis of paraffin-embedded rat testis using ELSPBP1 Rabbit pAb (A15882) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - ELSPBP1 Rabbit pAb (A15882)

Immunofluorescence analysis of paraffin-embedded mouse testis using ELSPBP1 Rabbit pAb (A15882) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameELSPBP1 Rabbit pAb
Catalog No.A15882
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene belongs to the sperm-coating protein family of epididymal origin. This protein and its canine homolog are the first known examples of proteins with four tandemly arranged fibronectin type 2 (Fn2) domains in the Fn2-module protein family.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 110-223 of human ELSPBP1 (NP_071425.3).
SequenceQQWKFCETNEYGGNSLRKPCIFPSIYRNNVVSDCMEDESNKLWCPTTENMDKDGKWSFCADTRISALVPGFPCHFPFNYKNKNYFNCTNEGSKENLVWCATSYNYDQDHTWVYC
Gene ID
Swiss Prot
SynonymsE12; HE12; EL149; EDDM12; ELSPBP1
Calculated MW26kDa
Observed MW26kDa
ReactivityMouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesMouse testis, Rat testis
Cellular locationSecreted

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

    ABclonal:Western blot - ELSPBP1 Rabbit pAb (A15882)}

    Western blot - ELSPBP1 Rabbit pAb (A15882)

    Western blot analysis of various lysates using ELSPBP1 Rabbit pAb (A15882) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 90s.
    ABclonal:Immunofluorescence - ELSPBP1 Rabbit pAb (A15882)}

    Immunofluorescence - ELSPBP1 Rabbit pAb (A15882)

    Immunofluorescence analysis of paraffin-embedded rat testis using ELSPBP1 Rabbit pAb (A15882) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - ELSPBP1 Rabbit pAb (A15882)}

    Immunofluorescence - ELSPBP1 Rabbit pAb (A15882)

    Immunofluorescence analysis of paraffin-embedded mouse testis using ELSPBP1 Rabbit pAb (A15882) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

    항체 (1)

    Secondary Antibodies (26)