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ENOX2 Rabbit pAb (A7063)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - ENOX2 Rabbit pAb (A7063)

Western blot analysis of extracts of Mouse lung, using ENOX2 antibody (A7063) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Western blot - ENOX2 Rabbit pAb (A7063)

Western blot analysis of extracts of Rat lung, using ENOX2 antibody (A7063) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunofluorescence - ENOX2 Rabbit pAb (A7063)

Immunofluorescence analysis of C6 cells using ENOX2 antibody (A7063) at dilution of 1:100. Blue: DAPI for nuclear staining.

Overview

Product nameENOX2 Rabbit pAb
Catalog No.A7063
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene is a tumor-specific member of the ECTO-NOX family of genes that encode cell surface NADH oxidases. The encoded protein has two enzymatic activities: catalysis of hydroquinone or NADH oxidation, and protein disulfide interchange. The protein also displays prion-like properties. Alternative splicing results in multiple transcript variants.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 411-610 of human ENOX2 (NP_872114.1).
SequenceAEALKEENDSLRWQLDAYRNEVELLKQEQGKVHREDDPNKEQQLKLLQQALQGMQQHLLKVQEEYKKKEAELEKLKDDKLQVEKMLENLKEKESCASRLCASNQDSEYPLEKTMNSSPIKSEREALLVGIISTFLHVHPFGASIEYICSYLHRLDNKICTSDVECLMGRLQHTFKQEMTGVGASLEKRWKFCGFEGLKLT
Gene ID
Swiss Prot
SynonymsAPK1; tNOX; COVA1
Calculated MW70kDa
Observed MW70kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesMouse lung, Rat lung
Cellular locationCell membrane, Secreted, extracellular space

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - ENOX2 Rabbit pAb (A7063)}

    Western blot - ENOX2 Rabbit pAb (A7063)

    Western blot analysis of extracts of Mouse lung, using ENOX2 antibody (A7063) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 1s.
    ABclonal:Western blot - ENOX2 Rabbit pAb (A7063)}

    Western blot - ENOX2 Rabbit pAb (A7063)

    Western blot analysis of extracts of Rat lung, using ENOX2 antibody (A7063) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 1s.
    ABclonal:Immunofluorescence - ENOX2 Rabbit pAb (A7063)}

    Immunofluorescence - ENOX2 Rabbit pAb (A7063)

    Immunofluorescence analysis of C6 cells using ENOX2 antibody (A7063) at dilution of 1:100. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

    항체 (2)

    Secondary Antibodies (26)