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ENPEP Rabbit pAb (A6905)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - ENPEP Rabbit pAb (A6905)

Western blot analysis of lysates from mouse lung, using ENPEP Rabbit pAb (A6905) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.

ABclonal:Immunohistochemistry - ENPEP Rabbit pAb (A6905)

Immunohistochemistry analysis of paraffin-embedded Rat kidney using ENPEP Rabbit pAb (A6905) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - ENPEP Rabbit pAb (A6905)

Immunohistochemistry analysis of paraffin-embedded Human prostate using ENPEP Rabbit pAb (A6905) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - ENPEP Rabbit pAb (A6905)

Immunohistochemistry analysis of paraffin-embedded Human stomach using ENPEP Rabbit pAb (A6905) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Overview

Product nameENPEP Rabbit pAb
Catalog No.A6905
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The ENPEP gene encodes glutamyl aminopeptidase, a type II integral membrane protein with an extracellular zinc-binding domain. This protein can upregulate blood pressure by cleaving the N-terminal aspartate from angiotensin II, and can regulate blood vessel formation and enhance tumorigenesis in some tissues. Along with ANPEP and DPP4, ENPEP was found to be a candidate co-receptor for the coronavirus SARS-CoV-2, which causes COVID-19.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 708-957 of human ENPEP (NP_001968.3).
SequenceDDKELYPMIEEYFQGQVKPIADSLGWNDAGDHVTKLLRSSVLGFACKMGDREALNNASSLFEQWLNGTVSLPVNLRLLVYRYGMQNSGNEISWNYTLEQYQKTSLAQEKEKLLYGLASVKNVTLLSRYLDLLKDTNLIKTQDVFTVIRYISYNSYGKNMAWNWIQLNWDYLVNRYTLNNRNLGRIVTIAEPFNTELQLWQMESFFAKYPQAGAGEKPREQVLETVKNNIEWLKQHRNTIREWFFNLLESG
Gene ID
Swiss Prot
SynonymsAPA; CD249; gp160; ENPEP
Calculated MW109kDa
Observed MW109-160kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesMouse lung
Cellular locationMembrane, Single-pass type II membrane protein

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ABclonal:Western blot - ENPEP Rabbit pAb (A6905)}

Western blot - ENPEP Rabbit pAb (A6905)

Western blot analysis of lysates from mouse lung, using ENPEP Rabbit pAb (A6905) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.
ABclonal:Immunohistochemistry - ENPEP Rabbit pAb (A6905)}

Immunohistochemistry - ENPEP Rabbit pAb (A6905)

Immunohistochemistry analysis of paraffin-embedded Rat kidney using ENPEP Rabbit pAb (A6905) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - ENPEP Rabbit pAb (A6905)}

Immunohistochemistry - ENPEP Rabbit pAb (A6905)

Immunohistochemistry analysis of paraffin-embedded Human prostate using ENPEP Rabbit pAb (A6905) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - ENPEP Rabbit pAb (A6905)}

Immunohistochemistry - ENPEP Rabbit pAb (A6905)

Immunohistochemistry analysis of paraffin-embedded Human stomach using ENPEP Rabbit pAb (A6905) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

* For research use only. Not for therapeutic or diagnostic purposes.

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Secondary Antibodies (26)