제품 > 항체 > 단일클론항체(mAb)

ENPP3/CD203c Rabbit mAb (A24649)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human

Western blot analysis of lysates from wild type (WT) and 293F cells transfected with ENPP3/CD203c Rabbit pAb, using ENPP3/CD203c Rabbit pAb (A24649) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunofluorescence - ENPP3/CD203c Rabbit mAb (A24649)

Confocal imaging of 293F cells using ENPP3/CD203c Rabbit mAb (A24649, at dilution of 1:200) (Red). DAPI was used for nuclear staining (blue). Objective: 100x.

ABclonal:Flow CytoMetry - ENPP3/CD203c Rabbit mAb (A24649)

Flow cytometry: 1X10^6 293F cells (negative control, left) and 293F (Transfection, right) cells were surface-stained with ENPP3/CD203c Rabbit mAb (A24649, 2 μg/mL, orange line) or ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, blue line), followed by Alexa Fluor® 647 conjugated goat anti-rabbit pAb staining. Non-fluorescently stained cells were used as blank control (red line).

Flow cytometry: 1X10^6 293F (Transfection) cells were surface-stained with ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, left) or ENPP3/CD203c Rabbit mAb (A24649, 2 μg/mL, right).

Overview

Product name	ENPP3/CD203c Rabbit mAb
Catalog No.	A24649
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC63726

Background

The protein encoded by this gene belongs to a series of ectoenzymes that are involved in hydrolysis of extracellular nucleotides. These ectoenzymes possess ATPase and ATP pyrophosphatase activities and are type II transmembrane proteins. Expression of the related rat mRNA has been found in a subset of immature glial cells and in the alimentary tract. The corresponding rat protein has been detected in the pancreas, small intestine, colon, and liver. The human mRNA is expressed in glioma cells, prostate, and uterus. Expression of the human protein has been detected in uterus, basophils, and mast cells. Two transcript variants, one protein coding and the other non-protein coding, have been found for this gene.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 551-875 of human ENPP3/CD203c (NP_005012.2).
Sequence	SHAEEVSKFSVCGFANPLPTESLDCFCPHLQNSTQLEQVNQMLNLTQEEITATVKVNLPFGRPRVLQKNVDHCLLYHREYVSGFGKAMRMPMWSSYTVPQLGDTSPLPPTVPDCLRADVRVPPSESQKCSFYLADKNITHGFLYPPASNRTSDSQYDALITSNLVPMYEEFRKMWDYFHSVLLIKHATERNGVNVVSGPIFDYNYDGHFDAPDEITKHLANTDVPIPTHYFVVLTSCKNKSHTPENCPGWLDVLPFIIPHRPTNVESCPEGKPEALWVEERFTAHIARVRDVELLTGLDFYQDKVQPVSEILQLKTYLPTFETTI
Gene ID
Swiss Prot
Synonyms	B10; NPP3; PDNP3; CD203c; PD-IBETA; ENPP3/CD203c
Calculated MW	100kDa
Observed MW	140kDa

Applications

Reactivity	Human
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 IF/ICC 1:50 - 1:200 FC 1:500 - 1:1000 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting Immunofluorescence Flow Cytometry
Positive samples	293F transfected with ENPP3 (Human)
Cellular location	Membrane, secreted, single-pass type II membrane protein.

Documents

Certificate of Compliance

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Lot number

Research Area

연구면역학 & 염증CDs

ENPP3/CD203c Rabbit mAb images

Western blot - ENPP3/CD203c Rabbit mAb (A24649)

Immunofluorescence - ENPP3/CD203c Rabbit mAb (A24649)

Confocal imaging of 293F cells using ENPP3/CD203c Rabbit mAb (A24649, at dilution of 1:200) (Red). DAPI was used for nuclear staining (blue). Objective: 100x.

Flow CytoMetry - ENPP3/CD203c Rabbit mAb (A24649)

Flow cytometry: 1X10^6 293F (Transfection) cells were surface-stained with ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, left) or ENPP3/CD203c Rabbit mAb (A24649, 2 μg/mL, right).

* For research use only. Not for therapeutic or diagnostic purposes.