제품 > 항체 > 단일클론항체(mAb)

ENPP5 Rabbit mAb (A9415)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - ENPP5 Rabbit mAb (A9415)

Western blot analysis of extracts of various cell lines, using (A9415) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Western blot - ENPP5 Rabbit mAb (A9415)

Western blot analysis of extracts of various cell lines, using (A9415) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.

ABclonal:Immunofluorescence - ENPP5 Rabbit mAb (A9415)

Immunofluorescence analysis of Neuro-2a cells using ENPP5 Rabbit mAb (A9415) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - ENPP5 Rabbit mAb (A9415)

Immunofluorescence analysis of SH-SY5Y cells using ENPP5 Rabbit mAb (A9415) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Overview

Product nameENPP5 Rabbit mAb
Catalog No.A9415
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC2780
This gene encodes a type-I transmembrane glycoprotein. Studies in rat suggest the encoded protein may play a role in neuronal cell communications. Alternatively spliced transcript variants have been described.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 378-477 of human ENPP5 (Q9UJA9).
SequenceCHLLNITAMPHNGSFWNVQDLLNSAMPRVVPYTQSTILLPGSVKPAEYDQEGSYPYFIGVSLGSIIVIVFFVIFIKHLIHSQIPALQDMHAEIAQPLLQA
Gene ID
Swiss Prot
SynonymsNPP5; NPP-5
Calculated MW55kDa
Observed MW54kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesSH-SY5Y, Y79, Mouse brain, Rat brain
Cellular locationextracellular region, plasma membrane

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - ENPP5 Rabbit mAb (A9415)}

    Western blot - ENPP5 Rabbit mAb (A9415)

    Western blot analysis of extracts of various cell lines, using (A9415) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 10s.
    ABclonal:Western blot - ENPP5 Rabbit mAb (A9415)}

    Western blot - ENPP5 Rabbit mAb (A9415)

    Western blot analysis of extracts of various cell lines, using (A9415) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Enhanced Kit (RM00021).
    Exposure time: 90s.
    ABclonal:Immunofluorescence - ENPP5 Rabbit mAb (A9415)}

    Immunofluorescence - ENPP5 Rabbit mAb (A9415)

    Immunofluorescence analysis of Neuro-2a cells using ENPP5 Rabbit mAb (A9415) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - ENPP5 Rabbit mAb (A9415)}

    Immunofluorescence - ENPP5 Rabbit mAb (A9415)

    Immunofluorescence analysis of SH-SY5Y cells using ENPP5 Rabbit mAb (A9415) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.