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EPOR Rabbit pAb (A2917)

Publications (3) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - EPOR Rabbit pAb (A2917)

Western blot analysis of lysates from Mouse liver, using EPOR Rabbit pAb (A2917) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Immunofluorescence - EPOR Rabbit pAb (A2917)

Immunofluorescence analysis of paraffin-embedded Mouse fetal using EPOR Rabbit pAb (A2917) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - EPOR Rabbit pAb (A2917)

Immunofluorescence analysis of NIH/3T3 cells using EPOR Rabbit pAb (A2917) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - EPOR Rabbit pAb (A2917)

Immunofluorescence analysis of PC-12 cells using EPOR Rabbit pAb (A2917) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - EPOR Rabbit pAb (A2917)

Immunofluorescence analysis of SH-SY5Y cells using EPOR Rabbit pAb (A2917) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameEPOR Rabbit pAb
Catalog No.A2917
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes the erythropoietin receptor which is a member of the cytokine receptor family. Upon erythropoietin binding, this receptor activates Jak2 tyrosine kinase which activates different intracellular pathways including: Ras/MAP kinase, phosphatidylinositol 3-kinase and STAT transcription factors. The stimulated erythropoietin receptor appears to have a role in erythroid cell survival. Defects in the erythropoietin receptor may produce erythroleukemia and familial erythrocytosis. Dysregulation of this gene may affect the growth of certain tumors. Alternate splicing results in multiple transcript variants.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 274-508 of human EPOR (NP_000112.1).
SequenceHRRALKQKIWPGIPSPESEFEGLFTTHKGNFQLWLYQNDGCLWWSPCTPFTEDPPASLEVLSERCWGTMQAVEPGTDDEGPLLEPVGSEHAQDTYLVLDKWLLPRNPPSEDLPGPGGSVDIVAMDEGSEASSCSSALASKPSPEGASAASFEYTILDPSSQLLRPWTLCPELPPTPPHLKYLYLVVSDSGISTDYSSGDSQGAQGGLSDGPYSNPYENSLIPAAEPLPPSYVACS
Gene ID
Swiss Prot
SynonymsEPO-R; EPOR
Calculated MW55kDa
Observed MW60kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesMouse liver
Cellular locationCell membrane, Secreted, Single-pass type I membrane protein.
Customer validation

WB(Rattus norvegicus, Homo sapiens)

IHC(Homo sapiens)

IF(Homo sapiens)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

ABclonal:Western blot - EPOR Rabbit pAb (A2917)}

Western blot - EPOR Rabbit pAb (A2917)

Western blot analysis of lysates from Mouse liver, using EPOR Rabbit pAb (A2917) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Immunofluorescence - EPOR Rabbit pAb (A2917)}

Immunofluorescence - EPOR Rabbit pAb (A2917)

Immunofluorescence analysis of paraffin-embedded Mouse fetal using EPOR Rabbit pAb (A2917) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - EPOR Rabbit pAb (A2917)}

Immunofluorescence - EPOR Rabbit pAb (A2917)

Immunofluorescence analysis of NIH/3T3 cells using EPOR Rabbit pAb (A2917) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - EPOR Rabbit pAb (A2917)}

Immunofluorescence - EPOR Rabbit pAb (A2917)

Immunofluorescence analysis of PC-12 cells using EPOR Rabbit pAb (A2917) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - EPOR Rabbit pAb (A2917)}

Immunofluorescence - EPOR Rabbit pAb (A2917)

Immunofluorescence analysis of SH-SY5Y cells using EPOR Rabbit pAb (A2917) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A2917? Please let us know so that we can cite the reference in this datasheet.

ELISA 키트 (1)

재조합 단백질 (1)

Secondary Antibodies (26)