제품 > 항체 > 다중클론항체(pAb)

ERLIN1 Rabbit pAb (A14843)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates using ERLIN1 Rabbit pAb (A14843) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - ERLIN1 Rabbit pAb (A14843)

Immunohistochemistry analysis of paraffin-embedded human colon using ERLIN1 Rabbit pAb (A14843) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunoprecipitation - ERLIN1 Rabbit pAb (A14843)

Immunoprecipitation analysis of 300 μg extracts of DU145 cells using 3 μg ERLIN1 antibody (A14843). Western blot was performed from the immunoprecipitate using ERLIN1 antibody (A14843) at a dilution of 1:1000.

Overview

Product name	ERLIN1 Rabbit pAb
Catalog No.	A14843
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

The protein encoded by this gene is part of a protein complex that mediates degradation of inositol 1, 4, 5-trisphosphate receptors in the endoplasmic reticulum. The encoded protein also binds cholesterol and regulates the SREBP signaling pathway, which promotes cellular cholesterol homeostasis. Defects in this gene have been associated with spastic paraplegia 62.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 259-348 of human ERLIN1 (NP_006450.2).
Sequence	KADAEYYAAHKYATSNKHKLTPEYLELKKYQAIASNSKIYFGSNIPNMFVDSSCALKYSDIRTGRESSLPSKEALEPSGENVIQNKESTG
Gene ID
Swiss Prot
Synonyms	KE04; KEO4; SPFH1; SPG62; Erlin-1; C10orf69; ERLIN1
Calculated MW	39kDa
Observed MW	39kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000 IHC-P 1:50 - 1:200 IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry Immunoprecipitation
Positive samples	293T, HepG2, BxPC-3, DU145, OVCAR3, Mouse kidney, Rat testis
Cellular location	Endoplasmic reticulum membrane, Single-pass type II membrane protein