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ETFA Rabbit pAb (A7670)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - ETFA Rabbit pAb (A7670)

Western blot analysis of various lysates using ETFA Rabbit pAb (A7670) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates / proteins: 25 μg per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Western blot - ETFA Rabbit pAb (A7670)

Western blot analysis of various lysates using ETFA Rabbit pAb (A7670) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates / proteins: 25 μg per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - ETFA Rabbit pAb (A7670)

Immunohistochemistry analysis of paraffin-embedded Rat brain using ETFA Rabbit pAb (A7670) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - ETFA Rabbit pAb (A7670)

Immunohistochemistry analysis of paraffin-embedded Rat kidney using ETFA Rabbit pAb (A7670) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - ETFA Rabbit pAb (A7670)

Immunohistochemistry analysis of paraffin-embedded Human liver cancer using ETFA Rabbit pAb (A7670) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - ETFA Rabbit pAb (A7670)

Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer using ETFA Rabbit pAb (A7670) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - ETFA Rabbit pAb (A7670)

Immunohistochemistry analysis of paraffin-embedded Human esophageal cancer using ETFA Rabbit pAb (A7670) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - ETFA Rabbit pAb (A7670)

Immunohistochemistry analysis of paraffin-embedded Mouse spleen using ETFA Rabbit pAb (A7670) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunoprecipitation - ETFA Rabbit pAb (A7670)

Immunoprecipitation analysis of 300 μg extracts of 293T cells using 3 μg ETFA antibody (A7670). Western blot was performed from the immunoprecipitate using ETFA antibody (A7670) at a dilution of 1:1000.

Overview

Product nameETFA Rabbit pAb
Catalog No.A7670
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
ETFA participates in catalyzing the initial step of the mitochondrial fatty acid beta-oxidation. It shuttles electrons between primary flavoprotein dehydrogenases and the membrane-bound electron transfer flavoprotein ubiquinone oxidoreductase. Defects in electron-transfer-flavoprotein have been implicated in type II glutaricaciduria in which multiple acyl-CoA dehydrogenase deficiencies result in large excretion of glutaric, lactic, ethylmalonic, butyric, isobutyric, 2-methyl-butyric, and isovaleric acids. Two transcript variants encoding different isoforms have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-333 of human ETFA (NP_000117.1).
SequenceMFRAAAPGQLRRAASLLRFQSTLVIAEHANDSLAPITLNTITAATRLGGEVSCLVAGTKCDKVAQDLCKVAGIAKVLVAQHDVYKGLLPEELTPLILATQKQFNYTHICAGASAFGKNLLPRVAAKLEVAPISDIIAIKSPDTFVRTIYAGNALCTVKCDEKVKVFSVRGTSFDAAATSGGSASSEKASSTSPVEISEWLDQKLTKSDRPELTGAKVVVSGGRGLKSGENFKLLYDLADQLHAAVGASRAAVDAGFVPNDMQVGQTGKIVAPELYIAVGISGAIQHLAGMKDSKTIVAINKDPEAPIFQVADYGIVADLFKVVPEMTEILKKK
Gene ID
Swiss Prot
SynonymsEMA; GA2; MADD; ETFA
Calculated MW35kDa
Observed MW35kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
  • IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunoprecipitation    
Positive samplesMouse heart, Rat heart, Mouse liver, Rat liver
Cellular locationMitochondrion matrix

Documents

Certificate of Compliance

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ABclonal:Western blot - ETFA Rabbit pAb (A7670)}

Western blot - ETFA Rabbit pAb (A7670)

Western blot analysis of various lysates using ETFA Rabbit pAb (A7670) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates / proteins: 25 μg per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Western blot - ETFA Rabbit pAb (A7670)}

Western blot - ETFA Rabbit pAb (A7670)

Western blot analysis of various lysates using ETFA Rabbit pAb (A7670) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates / proteins: 25 μg per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunohistochemistry - ETFA Rabbit pAb (A7670)}

Immunohistochemistry - ETFA Rabbit pAb (A7670)

Immunohistochemistry analysis of paraffin-embedded Rat brain using ETFA Rabbit pAb (A7670) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - ETFA Rabbit pAb (A7670)}

Immunohistochemistry - ETFA Rabbit pAb (A7670)

Immunohistochemistry analysis of paraffin-embedded Rat kidney using ETFA Rabbit pAb (A7670) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - ETFA Rabbit pAb (A7670)}

Immunohistochemistry - ETFA Rabbit pAb (A7670)

Immunohistochemistry analysis of paraffin-embedded Human liver cancer using ETFA Rabbit pAb (A7670) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - ETFA Rabbit pAb (A7670)}

Immunohistochemistry - ETFA Rabbit pAb (A7670)

Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer using ETFA Rabbit pAb (A7670) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - ETFA Rabbit pAb (A7670)}

Immunohistochemistry - ETFA Rabbit pAb (A7670)

Immunohistochemistry analysis of paraffin-embedded Human esophageal cancer using ETFA Rabbit pAb (A7670) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - ETFA Rabbit pAb (A7670)}

Immunohistochemistry - ETFA Rabbit pAb (A7670)

Immunohistochemistry analysis of paraffin-embedded Mouse spleen using ETFA Rabbit pAb (A7670) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunoprecipitation - ETFA Rabbit pAb (A7670)}

Immunoprecipitation - ETFA Rabbit pAb (A7670)

Immunoprecipitation analysis of 300 μg extracts of 293T cells using 3 μg ETFA antibody (A7670). Western blot was performed from the immunoprecipitate using ETFA antibody (A7670) at a dilution of 1:1000.

* For research use only. Not for therapeutic or diagnostic purposes.

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Secondary Antibodies (26)