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ETHE1 Rabbit pAb (A10142)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - ETHE1 Rabbit pAb (A10142)

Western blot analysis of various lysates using ETHE1 Rabbit pAb (A10142) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

ABclonal:Western blot - ETHE1 Rabbit pAb (A10142)

Western blot analysis of various lysates using ETHE1 Rabbit pAb (A10142) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunohistochemistry - ETHE1 Rabbit pAb (A10142)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using ETHE1 Rabbit pAb (A10142) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunohistochemistry - ETHE1 Rabbit pAb (A10142)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney using ETHE1 Rabbit pAb (A10142) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunofluorescence - ETHE1 Rabbit pAb (A10142)

Immunofluorescence analysis of U-2 OS cells using ETHE1 Rabbit pAb (A10142) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameETHE1 Rabbit pAb
Catalog No.A10142
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the metallo beta-lactamase family of iron-containing proteins involved in the mitochondrial sulfide oxidation pathway. The encoded protein catalyzes the oxidation of a persulfide substrate to sulfite. Certain mutations in this gene cause ethylmalonic encephalopathy, an infantile metabolic disorder affecting the brain, gastrointestinal tract and peripheral vessels. Alternative splicing results in multiple transcript variants encoding different isoforms.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 8-245 of human ETHE1 (NP_055112.2).
SequenceVARRQLSQRGGSGAPILLRQMFEPVSCTFTYLLGDRESREAVLIDPVLETAPRDAQLIKELGLRLLYAVNTHCHADHITGSGLLRSLLPGCQSVISRLSGAQADLHIEDGDSIRFGRFALETRASPGHTPGCVTFVLNDHSMAFTGDALLIRGCGRTDFQQGCAKTLYHSVHEKIFTLPGDCLIYPAHDYHGFTVSTVEEERTLNPRLTLSCEEFVKIMGNLNLPKPQQIDFAVPANM
Gene ID
Swiss Prot
SynonymsHSCO; YF13H12; ETHE1
Calculated MW28kDa
Observed MW28kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHT-29, HepG2, Mouse large intestine, Mouse liver, Rat liver
Cellular locationCytoplasm, Mitochondrion matrix, Nucleus.
Customer validation

Cell transfection(Homo sapiens)

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - ETHE1 Rabbit pAb (A10142)}

Western blot - ETHE1 Rabbit pAb (A10142)

Western blot analysis of various lysates using ETHE1 Rabbit pAb (A10142) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
ABclonal:Western blot - ETHE1 Rabbit pAb (A10142)}

Western blot - ETHE1 Rabbit pAb (A10142)

Western blot analysis of various lysates using ETHE1 Rabbit pAb (A10142) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Immunohistochemistry - ETHE1 Rabbit pAb (A10142)}

Immunohistochemistry - ETHE1 Rabbit pAb (A10142)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using ETHE1 Rabbit pAb (A10142) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
ABclonal:Immunohistochemistry - ETHE1 Rabbit pAb (A10142)}

Immunohistochemistry - ETHE1 Rabbit pAb (A10142)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney using ETHE1 Rabbit pAb (A10142) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
ABclonal:Immunofluorescence - ETHE1 Rabbit pAb (A10142)}

Immunofluorescence - ETHE1 Rabbit pAb (A10142)

Immunofluorescence analysis of U-2 OS cells using ETHE1 Rabbit pAb (A10142) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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Secondary Antibodies (26)