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Engrailed 1 Rabbit pAb (A23060)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Immunofluorescence - Engrailed 1 Rabbit pAb (A23060)

Immunofluorescence analysis of A-431 cells using Engrailed 1 Rabbit pAb (A23060) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameEngrailed 1 Rabbit pAb
Catalog No.A23060
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Homeobox-containing genes are thought to have a role in controlling development. In Drosophila, the 'engrailed' (en) gene plays an important role during development in segmentation, where it is required for the formation of posterior compartments. Different mutations in the mouse homologs, En1 and En2, produced different developmental defects that frequently are lethal. The human engrailed homologs 1 and 2 encode homeodomain-containing proteins and have been implicated in the control of pattern formation during development of the central nervous system.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human Engrailed 1. (NP_001417.3).
SequenceMEEQQPEPKSQRDSALGAAAAATPGGLSLSLSPGASGSSGSGSDGDSVPVSPQPAPPSPPAAPCLPPLAHHPHLPPHPPPPPPQHLAAPAHQPQPAAQLH
Gene ID
Swiss Prot
SynonymsENDOVESLB; Engrailed 1
Calculated MW40kDa
Observed MWRefer to figures
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationImmunofluorescence    
Positive samples
Cellular locationnucleus

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ABclonal:Immunofluorescence - Engrailed 1 Rabbit pAb (A23060)}

Immunofluorescence - Engrailed 1 Rabbit pAb (A23060)

Immunofluorescence analysis of A-431 cells using Engrailed 1 Rabbit pAb (A23060) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

항체 (1)

Secondary Antibodies (26)