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Erlin-2 Rabbit mAb (A0781)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Erlin-2 Rabbit mAb (A0781)

Western blot analysis of various lysates using Erlin-2 Rabbit mAb (A0781) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunohistochemistry - Erlin-2 Rabbit mAb (A0781)

Immunohistochemistry analysis of paraffin-embedded mouse kidney using Erlin-2 Rabbit mAb (A0781) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - Erlin-2 Rabbit mAb (A0781)

Immunohistochemistry analysis of paraffin-embedded rat ovary using Erlin-2 Rabbit mAb (A0781) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Overview

Product nameErlin-2 Rabbit mAb
Catalog No.A0781
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC2538
This gene encodes a member of the SPFH domain-containing family of lipid raft-associated proteins. The encoded protein is localized to lipid rafts of the endoplasmic reticulum and plays a critical role in inositol 1, 4, 5-trisphosphate (IP3) signaling by mediating ER-associated degradation of activated IP3 receptors. Mutations in this gene are a cause of spastic paraplegia-18 (SPG18). Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 50-150 of human Erlin-2 (O94905).
SequenceHLMLPFITSYKSVQTTLQTDEVKNVPCGTSGGVMIYFDRIEVVNFLVPNAVYDIVKNYTADYDKALIFNKIHHELNQFCSVHTLQEVYIELFDQIDENLKL
Gene ID
Swiss Prot
SynonymsNET32; SPFH2; SPG18; C8orf2; Erlin-2
Calculated MW38kDa
Observed MW43kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samples293T, PC-3, OVCAR3, C2C12
Cellular locationEndoplasmic reticulum membrane, Single-pass type II membrane protein

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ABclonal:Western blot - Erlin-2 Rabbit mAb (A0781)}

Western blot - Erlin-2 Rabbit mAb (A0781)

Western blot analysis of various lysates using Erlin-2 Rabbit mAb (A0781) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunohistochemistry - Erlin-2 Rabbit mAb (A0781)}

Immunohistochemistry - Erlin-2 Rabbit mAb (A0781)

Immunohistochemistry analysis of paraffin-embedded mouse kidney using Erlin-2 Rabbit mAb (A0781) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - Erlin-2 Rabbit mAb (A0781)}

Immunohistochemistry - Erlin-2 Rabbit mAb (A0781)

Immunohistochemistry analysis of paraffin-embedded rat ovary using Erlin-2 Rabbit mAb (A0781) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

* For research use only. Not for therapeutic or diagnostic purposes.

Secondary Antibodies (26)