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Exportin 5 (XPO5) Rabbit pAb (A6790)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Exportin 5 (XPO5) Rabbit pAb (A6790)

Western blot analysis of various lysates, using Exportin 5 (XPO5) Rabbit pAb (A6790) at 1:10000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunofluorescence - Exportin 5 (XPO5) Rabbit pAb (A6790)

Immunofluorescence analysis of MCF7 cells using Exportin 5 (XPO5) Rabbit pAb (A6790).Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution.

ABclonal:Immunofluorescence - Exportin 5 (XPO5) Rabbit pAb (A6790)

Immunofluorescence analysis of MCF7 cells using Exportin 5 (XPO5) Rabbit pAb (A6790) at dilution of 1:500 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Exportin 5 (XPO5) Rabbit pAb (A6790)

Immunofluorescence analysis of PC-12 cells using Exportin 5 (XPO5) Rabbit pAb (A6790) at dilution of 1:500 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - Exportin 5 (XPO5) Rabbit pAb (A6790)

Immunoprecipitation analysis of 200 μg extracts of HeLa cells using 1 μg Exportin 5 (Exportin 5 (XPO5)) antibody (A6790). Western blot was performed from the immunoprecipitate using Exportin 5 (Exportin 5 (XPO5)) antibody (A6790) at a dilution of 1:1000.

Overview

Product nameExportin 5 (XPO5) Rabbit pAb
Catalog No.A6790
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the karyopherin family that is required for the transport of small RNAs and double-stranded RNA-binding proteins from the nucleus to the cytoplasm. The encoded protein translocates cargo through the nuclear pore complex in a RanGTP-dependent process.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 925-1204 of human Exportin 5 (Exportin 5 (XPO5)) (NP_065801.1).
SequenceMRLSQKWQVINQRSLLCGEDEAADENPESQEMLEEQLVRMLTREVMDLITVCCVSKKGADHSSAPPADGDDEEMMATEVTPSAMAELTDLGKCLMKHEDVCTALLITAFNSLAWKDTLSCQRTTSQLCWPLLKQVLSGTLLADAVTWLFTSVLKGLQMHGQHDGCMASLVHLAFQIYEALRPRYLEIRAVMEQIPEIQKDSLDQFDCKLLNPSLQKVADKRRKDQFKRLIAGCIGKPLGEQFRKEVHIKNLPSLFKKTKPMLETEVLDNDGGGLATIFEP
Gene ID
Swiss Prot
Synonymsexp5; Exportin 5 (XPO5)
Calculated MW136kDa
Observed MW136kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:2000 - 1:10000
  • IF/ICC 1:100 - 1:500
  • IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    Immunoprecipitation    
Positive samplesK-562
Cellular locationCytoplasm, Nucleus

Documents

Certificate of Compliance

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ABclonal:Western blot - Exportin 5 (XPO5) Rabbit pAb (A6790)}

Western blot - Exportin 5 (XPO5) Rabbit pAb (A6790)

Western blot analysis of various lysates, using Exportin 5 (XPO5) Rabbit pAb (A6790) at 1:10000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunofluorescence - Exportin 5 (XPO5) Rabbit pAb (A6790)}

Immunofluorescence - Exportin 5 (XPO5) Rabbit pAb (A6790)

Immunofluorescence analysis of MCF7 cells using Exportin 5 (XPO5) Rabbit pAb (A6790).Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution.
ABclonal:Immunofluorescence - Exportin 5 (XPO5) Rabbit pAb (A6790)}

Immunofluorescence - Exportin 5 (XPO5) Rabbit pAb (A6790)

Immunofluorescence analysis of MCF7 cells using Exportin 5 (XPO5) Rabbit pAb (A6790) at dilution of 1:500 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Exportin 5 (XPO5) Rabbit pAb (A6790)}

Immunofluorescence - Exportin 5 (XPO5) Rabbit pAb (A6790)

Immunofluorescence analysis of PC-12 cells using Exportin 5 (XPO5) Rabbit pAb (A6790) at dilution of 1:500 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunoprecipitation - Exportin 5 (XPO5) Rabbit pAb (A6790)}

Immunoprecipitation - Exportin 5 (XPO5) Rabbit pAb (A6790)

Immunoprecipitation analysis of 200 μg extracts of HeLa cells using 1 μg Exportin 5 (Exportin 5 (XPO5)) antibody (A6790). Western blot was performed from the immunoprecipitate using Exportin 5 (Exportin 5 (XPO5)) antibody (A6790) at a dilution of 1:1000.

* For research use only. Not for therapeutic or diagnostic purposes.

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Secondary Antibodies (26)