제품 > 항체 > 다중클론항체(pAb)

Ezrin Rabbit pAb (A0703)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates using Ezrin Rabbit pAb (A0703) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunofluorescence - Ezrin Rabbit pAb (A0703)

Immunofluorescence analysis of C6 cells using Ezrin Rabbit pAb (A0703) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U-2 OS cells using Ezrin Rabbit pAb (A0703) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - Ezrin Rabbit pAb (A0703)

Immunoprecipitation analysis of 200 μg extracts of 293T cells, using 3 μg Ezrinin antibody (A0703). Western blot was performed from the immunoprecipitate using Ezrinin antibody (A0703) at a dilution of 1:1000.

Overview

Product name	Ezrin Rabbit pAb
Catalog No.	A0703
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

The cytoplasmic peripheral membrane protein encoded by this gene functions as a protein-tyrosine kinase substrate in microvilli. As a member of the ERM protein family, this protein serves as an intermediate between the plasma membrane and the actin cytoskeleton. This protein plays a key role in cell surface structure adhesion, migration and organization, and it has been implicated in various human cancers. A pseudogene located on chromosome 3 has been identified for this gene. Alternatively spliced variants have also been described for this gene.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 350-470 of human Ezrinin (NP_003370.2).
Sequence	RLQDYEEKTKKAERELSEQIQRALQLEEERKRAQEEAERLEADRMAALRAKEELERQAVDQIKSQEQLAAELAEYTAKIALLEEARRRKEDEVEEWQHRAKEAQDDLVKTKEELHLVMTAP
Gene ID
Swiss Prot
Synonyms	CVL; CVIL; VIL2; HEL-S-105; Ezrin
Calculated MW	69kDa
Observed MW	90kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000 IF/ICC 1:50 - 1:100 IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting Immunofluorescence Immunoprecipitation
Positive samples	M21, SW480, U-251MG, 293T, Mouse intestine, Mouse brain, Rat brain
Cellular location	Apical cell membrane, Cell projection, Cytoplasm, Cytoplasmic side, Peripheral membrane protein, cell cortex, cytoskeleton, microvillus membrane, ruffle membrane
Customer validation	WB(Mus musculus)