제품 > 항체 > 인산화 특이적 항체

Phospho-Ezrin-Y353 Rabbit pAb (AP1118)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human

Western blot analysis of lysates from 293T cells, using Phospho-Ezrin-Y353 Rabbit pAb (A0703). 293T cells were treated by ATP(5 mM) at 30℃ for 1 hour.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

Overview

Product name	Phospho-Ezrin-Y353 Rabbit pAb
Catalog No.	AP1118
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

The cytoplasmic peripheral membrane protein encoded by this gene functions as a protein-tyrosine kinase substrate in microvilli. As a member of the ERM protein family, this protein serves as an intermediate between the plasma membrane and the actin cytoskeleton. This protein plays a key role in cell surface structure adhesion, migration and organization, and it has been implicated in various human cancers. A pseudogene located on chromosome 3 has been identified for this gene. Alternatively spliced variants have also been described for this gene.

Immunogen information

Immunogen	A synthetic phosphorylated peptide around Y353 of human Ezrin (NP_003370.2).
Sequence	QDYEE
Gene ID
Swiss Prot
Synonyms	CVL; CVIL; VIL2; HEL-S-105; Phospho-Ezrin-Y353
Calculated MW	69kDa
Observed MW	70kDa

Applications

Reactivity	Human
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	293T treated by ATP
Cellular location	Apical cell membrane, Cell projection, Cytoplasm, Cytoplasmic side, Peripheral membrane protein, cell cortex, cytoskeleton, microvillus membrane, ruffle membrane.