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FABP5 Rabbit pAb (A6373)

Review (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - FABP5 Rabbit pAb (A6373)

Western blot analysis of various lysates using FABP5 Rabbit pAb (A6373) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - FABP5 Rabbit pAb (A6373)

Immunohistochemistry analysis of paraffin-embedded Human esophageal cancer using FABP5 Rabbit pAb (A6373) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - FABP5 Rabbit pAb (A6373)

Immunohistochemistry analysis of paraffin-embedded Human placenta using FABP5 Rabbit pAb (A6373) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - FABP5 Rabbit pAb (A6373)

Immunofluorescence analysis of HCT116 cells using FABP5 Rabbit pAb (A6373) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - FABP5 Rabbit pAb (A6373)

Immunofluorescence analysis of NIH/3T3 cells using FABP5 Rabbit pAb (A6373) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - FABP5 Rabbit pAb (A6373)

Immunofluorescence analysis of PC-12 cells using FABP5 Rabbit pAb (A6373) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - FABP5 Rabbit pAb (A6373)

Immunofluorescence analysis of U2OS cells using FABP5 Rabbit pAb (A6373) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Review
ABclonal: review for FABP5 Rabbit pAb(A6373)

Overview

Product nameFABP5 Rabbit pAb
Catalog No.A6373
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes the fatty acid binding protein found in epidermal cells, and was first identified as being upregulated in psoriasis tissue. Fatty acid binding proteins are a family of small, highly conserved, cytoplasmic proteins that bind long-chain fatty acids and other hydrophobic ligands. FABPs may play roles in fatty acid uptake, transport, and metabolism. Polymorphisms in this gene are associated with type 2 diabetes. The human genome contains many pseudogenes similar to this locus.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-135 of human FABP5 (NP_001435.1).
SequenceMATVQQLEGRWRLVDSKGFDEYMKELGVGIALRKMGAMAKPDCIITCDGKNLTIKTESTLKTTQFSCTLGEKFEETTADGRKTQTVCNFTDGALVQHQEWDGKESTITRKLKDGKLVVECVMNNVTCTRIYEKVE
Gene ID
Swiss Prot
SynonymsEFABP; KFABP; E-FABP; PAFABP; PA-FABP; FABP5
Calculated MW15kDa
Observed MW15kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHCT116, SH-SY5Y, Mouse liver, Rat liver, Rat brain, Rat stomach
Cellular locationCytoplasm.
Customer validation

(HCT-116 、PC-3、Ramos)

WB(Mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

ABclonal:Western blot - FABP5 Rabbit pAb (A6373)}

Western blot - FABP5 Rabbit pAb (A6373)

Western blot analysis of various lysates using FABP5 Rabbit pAb (A6373) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunohistochemistry - FABP5 Rabbit pAb (A6373)}

Immunohistochemistry - FABP5 Rabbit pAb (A6373)

Immunohistochemistry analysis of paraffin-embedded Human esophageal cancer using FABP5 Rabbit pAb (A6373) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - FABP5 Rabbit pAb (A6373)}

Immunohistochemistry - FABP5 Rabbit pAb (A6373)

Immunohistochemistry analysis of paraffin-embedded Human placenta using FABP5 Rabbit pAb (A6373) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunofluorescence - FABP5 Rabbit pAb (A6373)}

Immunofluorescence - FABP5 Rabbit pAb (A6373)

Immunofluorescence analysis of HCT116 cells using FABP5 Rabbit pAb (A6373) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - FABP5 Rabbit pAb (A6373)}

Immunofluorescence - FABP5 Rabbit pAb (A6373)

Immunofluorescence analysis of NIH/3T3 cells using FABP5 Rabbit pAb (A6373) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - FABP5 Rabbit pAb (A6373)}

Immunofluorescence - FABP5 Rabbit pAb (A6373)

Immunofluorescence analysis of PC-12 cells using FABP5 Rabbit pAb (A6373) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - FABP5 Rabbit pAb (A6373)}

Immunofluorescence - FABP5 Rabbit pAb (A6373)

Immunofluorescence analysis of U2OS cells using FABP5 Rabbit pAb (A6373) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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항체 (2)

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Secondary Antibodies (26)