제품 > 항체 > 다중클론항체(pAb)

FAM160B2 Rabbit pAb (A18836)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates using FAM160B2 Rabbit pAb (A18836) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - FAM160B2 Rabbit pAb (A18836)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney using FAM160B2 Rabbit pAb (A18836) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Rat spleen using FAM160B2 Rabbit pAb (A18836) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Rat testis using FAM160B2 Rabbit pAb (A18836) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - FAM160B2 Rabbit pAb (A18836)

Immunofluorescence analysis of NIH-3T3 cells using FAM160B2 Rabbit pAb (A18836) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - FAM160B2 Rabbit pAb (A18836)

Immunoprecipitation analysis of 600 μg extracts of Rat testis cells using 3 μg FAM160B2 antibody (A18836). Western blot was performed from the immunoprecipitate using FAM160B2 antibody (A18836) at a dilution of 1:1000.

Overview

Product name	FAM160B2 Rabbit pAb
Catalog No.	A18836
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

Orthologous to human FHIP2B (FHF complex subunit HOOK interacting protein 2B).

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 74-303 of mouse FAM160B2 (NP_919326.1).
Sequence	GEAGPCLEYLLQHKILETLCTLGKAEYPPGMRQQVFQFFSKVLSQVQHPLLHYLSVHRPVQKLLRLGGTVPGSLTEKEEVQFTSVLCSKIQQDPELLAYILEGKKIIGKKKTARESTAPPKDIAGYRDKDCPHSDALNRDPGLDKEHCGVPALSIHLPAETEGPENGPGESNLITSLLGLCKSKKSRLALKAQENILLLVSVASPAAATYLTQSTSCCMAIAEHLCQLYR
Gene ID
Swiss Prot
Synonyms	Rai16; Fam160b2; G430067P06Rik; FAM160B2
Calculated MW	82kDa
Observed MW	82kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 IHC-P 1:50 - 1:200 IF/ICC 1:50 - 1:200 IP 0.5μg-4μg antibody for 400μg-600μg extracts of whole cells ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry Immunofluorescence Immunoprecipitation
Positive samples	Mouse brain, Rat testis
Cellular location