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FAM20A Rabbit mAb (A22760)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - FAM20A Rabbit mAb (A22760)

Western blot analysis of various lysates, using FAM20A antibody (A22760) at 1:20000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.

Overview

Product nameFAM20A Rabbit mAb
Catalog No.A22760
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC58698
This locus encodes a protein that is likely secreted and may function in hematopoiesis. A mutation at this locus has been associated with amelogenesis imperfecta and gingival hyperplasia syndrome. Alternatively spliced transcript variants have been identified.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 500-600 of human FAM20A(NP_060035.2).
SequenceAIFDFLIGNMDRHHYEMFTKFGDDGFLIHLDNARGFGRHSHDEISILSPLSQCCMIKKKTLLHLQLLAQADYRLSDVMRESLLEDQLSPVLTEPHLLALDR
Gene ID
Swiss Prot
SynonymsAI1G; AIGFS; FP2747
Calculated MW61kDa
Observed MW59kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:2000 - 1:20000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesHep G2, A-431
Cellular locationEndoplasmic reticulum, Golgi apparatus, Secreted

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ABclonal:Western blot - FAM20A Rabbit mAb (A22760)}

Western blot - FAM20A Rabbit mAb (A22760)

Western blot analysis of various lysates, using FAM20A antibody (A22760) at 1:20000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.

* For research use only. Not for therapeutic or diagnostic purposes.

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