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FAM3A Rabbit pAb (A20686)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - FAM3A Rabbit pAb (A20686)

Western blot analysis of lysates from BxPC-3 cells using FAM3A Rabbit pAb(A20686) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunohistochemistry - FAM3A Rabbit pAb (A20686)

Immunohistochemistry analysis of paraffin-embedded human colon using FAM3A Rabbit pAb (A20686) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - FAM3A Rabbit pAb (A20686)

Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using FAM3A Rabbit pAb (A20686) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Overview

Product nameFAM3A Rabbit pAb
Catalog No.A20686
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a cytokine-like protein. The expression of this gene may be regulated by peroxisome proliferator-activated receptor gamma, and the encoded protein may be involved in the regulation of glucose and lipid metabolism. Alternative splicing results in multiple transcript variants.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 34-230 of human FAM3A (NP_068578.2).
SequenceFPRIQQLFTSPESSVTAAPRARKYKCGLPQPCPEEHLAFRVVSGAANVIGPKICLEDKMLMSSVKDNVGRGLNIALVNGVSGELIEARAFDMWAGDVNDLLKFIRPLHEGTLVFVASYDDPATKMNEETRKLFSELGSRNAKELAFRDSWVFVGAKGVQNKSPFEQHVKNSKHSNKYEGWPEALEMEGCIPRRSTAS
Gene ID
Swiss Prot
SynonymsDLD; 2.19; XAP-7; DXS560S; FAM3A
Calculated MW25kDa
Observed MW25kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesBxPC-3
Cellular locationSecreted

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - FAM3A Rabbit pAb (A20686)}

    Western blot - FAM3A Rabbit pAb (A20686)

    Western blot analysis of lysates from BxPC-3 cells using FAM3A Rabbit pAb(A20686) at 1:500 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25 μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 180s.
    ABclonal:Immunohistochemistry - FAM3A Rabbit pAb (A20686)}

    Immunohistochemistry - FAM3A Rabbit pAb (A20686)

    Immunohistochemistry analysis of paraffin-embedded human colon using FAM3A Rabbit pAb (A20686) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
    ABclonal:Immunohistochemistry - FAM3A Rabbit pAb (A20686)}

    Immunohistochemistry - FAM3A Rabbit pAb (A20686)

    Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using FAM3A Rabbit pAb (A20686) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

    * For research use only. Not for therapeutic or diagnostic purposes.

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    Secondary Antibodies (26)