제품 > 항체 > 다중클론항체(pAb)

FAM96B Rabbit pAb (A7806)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - FAM96B Rabbit pAb (A7806)

Western blot analysis of extracts of various cell lines, using FAM96B antibody (A7806) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 15s.

Overview

Product nameFAM96B Rabbit pAb
Catalog No.A7806
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Involved in chromosome segregation; iron-sulfur cluster assembly; and protein maturation by iron-sulfur cluster transfer. Located in cytosol; nucleoplasm; and spindle. Part of CIA complex and MMXD complex.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-163 of human FAM96B (NP_057146.1).
SequenceMVGGGGVGGGLLENANPLIYQRSGERPVTAGEEDEQVPDSIDAREIFDLIRSINDPEHPLTLEELNVVEQVRVQVSDPESTVAVAFTPTIPHCSMATLIGLSIKVKLLRSLPQRFKMDVHITPGTHASEHAVNKQLADKERVAAALENTHLLEVVNQCLSARS
Gene ID
Swiss Prot
SynonymsCIA2B; MIP18; FAM96B; CGI-128
Calculated MW18kDa
Observed MW18kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesHeLa, NCI-H460, MCF7, Mouse heart, Mouse testis
Cellular locationCytoplasm, Nucleus, cytoskeleton, spindle

Documents

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    ABclonal:Western blot - FAM96B Rabbit pAb (A7806)}

    Western blot - FAM96B Rabbit pAb (A7806)

    Western blot analysis of extracts of various cell lines, using FAM96B antibody (A7806) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 15s.

    * For research use only. Not for therapeutic or diagnostic purposes.