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FBXO32 Rabbit pAb (A25594)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Immunofluorescence - FBXO32 Rabbit pAb (A25594)

Immunofluorescence analysis of Mouse leg bone tissue using FBXO32 Rabbit pAb (A25594) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining.

ABclonal:Immunofluorescence - FBXO32 Rabbit pAb (A25594)

Immunofluorescence analysis of Rat leg bone tissue using FBXO32 Rabbit pAb (A25594) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining.

ABclonal:Immunofluorescence - FBXO32 Rabbit pAb (A25594)

Immunofluorescence analysis of A-431 cells using FBXO32 Rabbit pAb (A25594) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - FBXO32 Rabbit pAb (A25594)

Immunofluorescence analysis of HeLa cells using FBXO32 Rabbit pAb (A25594) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameFBXO32 Rabbit pAb
Catalog No.A25594
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class and contains an F-box domain. This protein is highly expressed during muscle atrophy, whereas mice deficient in this gene were found to be resistant to atrophy. This protein is thus a potential drug target for the treatment of muscle atrophy. Alternative splicing results in multiple transcript variants encoding different isoforms.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 201-300 of human FBXO32 (NP_478136.1).
SequenceETILHWQQQLNNIQITRPAFKGLTFTDLPLCLQLNIMQRLSDGRDLVSLGQAAPDLHVLSEDRLLWKKLCQYHFSERQIRKRLILSDKGQLDWKKMYFKL
Gene ID
Swiss Prot
SynonymsFbx32; MAFbx
Calculated MW42kDa
Observed MWRefer to figures
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key applicationImmunofluorescence    
Positive samples
Cellular locationCytoplasm, Nucleus.

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ABclonal:Immunofluorescence - FBXO32 Rabbit pAb (A25594)}

Immunofluorescence - FBXO32 Rabbit pAb (A25594)

Immunofluorescence analysis of Mouse leg bone tissue using FBXO32 Rabbit pAb (A25594) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining.
ABclonal:Immunofluorescence - FBXO32 Rabbit pAb (A25594)}

Immunofluorescence - FBXO32 Rabbit pAb (A25594)

Immunofluorescence analysis of Rat leg bone tissue using FBXO32 Rabbit pAb (A25594) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining.
ABclonal:Immunofluorescence - FBXO32 Rabbit pAb (A25594)}

Immunofluorescence - FBXO32 Rabbit pAb (A25594)

Immunofluorescence analysis of A-431 cells using FBXO32 Rabbit pAb (A25594) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - FBXO32 Rabbit pAb (A25594)}

Immunofluorescence - FBXO32 Rabbit pAb (A25594)

Immunofluorescence analysis of HeLa cells using FBXO32 Rabbit pAb (A25594) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

항체 (3)

Secondary Antibodies (26)