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FBXW11 Rabbit pAb (A20010)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - FBXW11 Rabbit pAb (A20010)

Western blot analysis of various lysates using FBXW11 Rabbit pAb (A20010) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunofluorescence - FBXW11 Rabbit pAb (A20010)

Immunofluorescence analysis of C6 cells using FBXW11 Rabbit pAb (A20010) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - FBXW11 Rabbit pAb (A20010)

Immunofluorescence analysis of NIH-3T3 cells using FBXW11 Rabbit pAb (A20010) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - FBXW11 Rabbit pAb (A20010)

Immunofluorescence analysis of U-2 OS cells using FBXW11 Rabbit pAb (A20010) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameFBXW11 Rabbit pAb
Catalog No.A20010
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbws class and, in addition to an F-box, contains multiple WD40 repeats. This gene contains at least 14 exons, and its alternative splicing generates 3 transcript variants diverging at the presence/absence of two alternate exons.
ImmunogenA synthetic Peptide of human FBXW11.
SequenceEmail for sequence
Gene ID
Swiss Prot
SynonymsHos; BTRC2; FBW1B; Fbw11; BTRCP2; FBXW1B; NEDJED; FBXW11
Calculated MW62kDa
Observed MW62kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesSH-SY5Y, A-431
Cellular locationCytoplasm, Nucleus

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ABclonal:Western blot - FBXW11 Rabbit pAb (A20010)}

Western blot - FBXW11 Rabbit pAb (A20010)

Western blot analysis of various lysates using FBXW11 Rabbit pAb (A20010) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunofluorescence - FBXW11 Rabbit pAb (A20010)}

Immunofluorescence - FBXW11 Rabbit pAb (A20010)

Immunofluorescence analysis of C6 cells using FBXW11 Rabbit pAb (A20010) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - FBXW11 Rabbit pAb (A20010)}

Immunofluorescence - FBXW11 Rabbit pAb (A20010)

Immunofluorescence analysis of NIH-3T3 cells using FBXW11 Rabbit pAb (A20010) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - FBXW11 Rabbit pAb (A20010)}

Immunofluorescence - FBXW11 Rabbit pAb (A20010)

Immunofluorescence analysis of U-2 OS cells using FBXW11 Rabbit pAb (A20010) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

항체 (1)

Secondary Antibodies (26)