제품 > 항체 > 다중클론항체(pAb)

FCHSD1 Rabbit pAb (A15543)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - FCHSD1 Rabbit pAb (A15543)

Western blot analysis of various lysates using FCHSD1 Rabbit pAb (A15543) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 15S.

ABclonal:Immunohistochemistry - FCHSD1 Rabbit pAb (A15543)

Immunohistochemistry analysis of paraffin-embedded Rat spleen using FCHSD1 Rabbit pAb (A15543) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - FCHSD1 Rabbit pAb (A15543)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney using FCHSD1 Rabbit pAb (A15543) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Overview

Product nameFCHSD1 Rabbit pAb
Catalog No.A15543
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Predicted to enable lipid binding activity. Predicted to be involved in neuromuscular synaptic transmission and positive regulation of actin filament polymerization. Predicted to be located in cell projection and perikaryon. Predicted to be active in neuromuscular junction and recycling endosome. Predicted to colocalize with cuticular plate.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 100-400 of human FCHSD1 (NP_258260.1).
SequenceRLQASDRYRDLAGGTGRSAKEQVLRKGTENLQRAQAEVLQSVRELSRSRKLYGQRERVWALAQEKAADVQARLNRSDHGIFHSRTSLQKLSTKLSAQSAQYSQQLQAARNEYLLNLVATNAHLDHYYQEELPALLKALVSELSEHLRDPLTSLSHTELEAAEVILEHAHRGEQTTSQVSWEQDLKLFLQEPGVFSPTPPQQFQPAGTDQVCVLEWGAEGVAGKSGLEKEVQRLTSRAARDYKIQNHGHRVLQRLEQRRQQASEREAPSIEQRLQEVRESIRRAQVSQVKGAARLALLQGAG
Gene ID
Swiss Prot
SynonymsNWK2; FCHSD1
Calculated MW77kDa
Observed MW75kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:200 - 1:2000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samples293T, HeLa, Jurkat, K-562, Mouse brain, Mouse liver, Rat brain, Rat liver
Cellular locationcell projection, neuromuscular junction, perikaryon, recycling endosome

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

    ABclonal:Western blot - FCHSD1 Rabbit pAb (A15543)}

    Western blot - FCHSD1 Rabbit pAb (A15543)

    Western blot analysis of various lysates using FCHSD1 Rabbit pAb (A15543) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 15S.
    ABclonal:Immunohistochemistry - FCHSD1 Rabbit pAb (A15543)}

    Immunohistochemistry - FCHSD1 Rabbit pAb (A15543)

    Immunohistochemistry analysis of paraffin-embedded Rat spleen using FCHSD1 Rabbit pAb (A15543) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
    ABclonal:Immunohistochemistry - FCHSD1 Rabbit pAb (A15543)}

    Immunohistochemistry - FCHSD1 Rabbit pAb (A15543)

    Immunohistochemistry analysis of paraffin-embedded Mouse kidney using FCHSD1 Rabbit pAb (A15543) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Secondary Antibodies (26)