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FDX1 Rabbit pAb (A24786)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - FDX1 Rabbit pAb (A24786)

Western blot analysis of lysates from Mouse testis using FDX1 Rabbit pAb(A24786) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 45s.

Overview

Product nameFDX1 Rabbit pAb
Catalog No.A24786
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a small iron-sulfur protein that transfers electrons from NADPH through ferredoxin reductase to mitochondrial cytochrome P450, involved in steroid, vitamin D, and bile acid metabolism. Pseudogenes of this functional gene are found on chromosomes 20 and 21.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 61-160 of human FDX1 (NP_004100.1).
SequenceSSSEDKITVHFINRDGETLTTKGKVGDSLLDVVVENNLDIDGFGACEGTLACSTCHLIFEDHIYEKLDAITDEENDMLDLAYGLTDRSRLGCQICLTKSM
Gene ID
Swiss Prot
SynonymsFDX1; ADX; FDX; LOH11CR1D; ferredoxin 1
Calculated MW19kDa
Observed MW14kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:5000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesMouse testis
Cellular locationMitochondrion matrix

Documents

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    ABclonal:Western blot - FDX1 Rabbit pAb (A24786)}

    Western blot - FDX1 Rabbit pAb (A24786)

    Western blot analysis of lysates from Mouse testis using FDX1 Rabbit pAb(A24786) at 1:2000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25 μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 45s.

    * For research use only. Not for therapeutic or diagnostic purposes.

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