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FGD1 Rabbit pAb (A16709)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Mouse, Rat

ABclonal:Western blot - FGD1 Rabbit pAb (A16709)

Western blot analysis of various lysates using FGD1 Rabbit pAb (A16709) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

Overview

Product nameFGD1 Rabbit pAb
Catalog No.A16709
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a protein that contains Dbl (DH) and pleckstrin (PH) homology domains and is similar to the Rho family of small GTP-binding proteins. The encoded protein specifically binds to the Rho family GTPase Cdc42Hs and can stimulate the GDP-GTP exchange of the isoprenylated form of Cdc42Hs. It also stimulates the mitogen activated protein kinase cascade leading to c-Jun kinase SAPK/JNK1 activation. Defects in this gene are the cause of the faciogenital dysplasia in Aarskog-Scott syndrome and a syndromatic form of X-linked cognitive disability.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 700-800 of human FGD1 (NP_004454.2).
SequenceLLNSTNREDEDTPPNSPNVDLGKRAPTPIREKEVTMCMRCQEPFNSITKRRHHCKACGHVVCGKCSEFRARLVYDNNRSNRVCTDCYVALHGVPGSSPACS
Gene ID
Swiss Prot
SynonymsAAS; FGDY; MRXS16; ZFYVE3; FGD1
Calculated MW107kDa
Observed MW110kDa
ReactivityMouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesmouse brain, mouse heart, rat brain
Cellular locationCell projection, Cytoplasm, cytoskeleton, lamellipodium, ruffle
ABclonal:Western blot - FGD1 Rabbit pAb (A16709)}

Western blot - FGD1 Rabbit pAb (A16709)

Western blot analysis of various lysates using FGD1 Rabbit pAb (A16709) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

* For research use only. Not for therapeutic or diagnostic purposes.

항체 (1)

Secondary Antibodies (26)