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FGF1 Rabbit pAb (A0685)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - FGF1 Rabbit pAb (A0685)

Western blot analysis of various lysates using FGF1 Rabbit pAb (A0685) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - FGF1 Rabbit pAb (A0685)

Immunohistochemistry analysis of paraffin-embedded Mouse brain using FGF1 Rabbit pAb (A0685) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunohistochemistry - FGF1 Rabbit pAb (A0685)

Immunohistochemistry analysis of paraffin-embedded Rat brain using FGF1 Rabbit pAb (A0685) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunofluorescence - FGF1 Rabbit pAb (A0685)

Immunofluorescence analysis of HepG2 cells using FGF1 Rabbit pAb (A0685) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameFGF1 Rabbit pAb
Catalog No.A0685
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein functions as a modifier of endothelial cell migration and proliferation, as well as an angiogenic factor. It acts as a mitogen for a variety of mesoderm- and neuroectoderm-derived cells in vitro, thus is thought to be involved in organogenesis. Multiple alternatively spliced variants encoding different isoforms have been described.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 16-155 of human FGF1 (NP_000791.1).
SequenceFNLPPGNYKKPKLLYCSNGGHFLRILPDGTVDGTRDRSDQHIQLQLSAESVGEVYIKSTETGQYLAMDTDGLLYGSQTPNEECLFLERLEENHYNTYISKKHAEKNWFVGLKKNGSCKRGPRTHYGQKAILFLPLPVSSD
Gene ID
Swiss Prot
SynonymsAFGF; ECGF; FGFA; ECGFA; ECGFB; FGF-1; HBGF1; HBGF-1; GLIO703; ECGF-beta; FGF-alpha; FGF1
Calculated MW17kDa
Observed MW17kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:100 - 1:200
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesU-251MG, Mouse heart, Rat heart
Cellular locationCytoplasm, Nucleus, Secreted, cell cortex, cytosol.

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - FGF1 Rabbit pAb (A0685)}

Western blot - FGF1 Rabbit pAb (A0685)

Western blot analysis of various lysates using FGF1 Rabbit pAb (A0685) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - FGF1 Rabbit pAb (A0685)}

Immunohistochemistry - FGF1 Rabbit pAb (A0685)

Immunohistochemistry analysis of paraffin-embedded Mouse brain using FGF1 Rabbit pAb (A0685) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
ABclonal:Immunohistochemistry - FGF1 Rabbit pAb (A0685)}

Immunohistochemistry - FGF1 Rabbit pAb (A0685)

Immunohistochemistry analysis of paraffin-embedded Rat brain using FGF1 Rabbit pAb (A0685) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
ABclonal:Immunofluorescence - FGF1 Rabbit pAb (A0685)}

Immunofluorescence - FGF1 Rabbit pAb (A0685)

Immunofluorescence analysis of HepG2 cells using FGF1 Rabbit pAb (A0685) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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