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FHIT Rabbit pAb (A1196)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - FHIT Rabbit pAb (A1196)

Western blot analysis of extracts of various cell lines, using FHIT antibody (A1196) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

Overview

Product nameFHIT Rabbit pAb
Catalog No.A1196
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a P1-P3-bis(5'-adenosyl) triphosphate hydrolase involved in purine metabolism. This gene encompasses the common fragile site FRA3B on chromosome 3, where carcinogen-induced damage can lead to translocations and aberrant transcripts. In fact, aberrant transcripts from this gene have been found in about half of all esophageal, stomach, and colon carcinomas. The encoded protein is also a tumor suppressor, as loss of its activity results in replication stress and DNA damage.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 25-147 of human FHIT (NP_001159715.1).
SequenceLVNRKPVVPGHVLVCPLRPVERFHDLRPDEVADLFQTTQRVGTVVEKHFHGTSLTFSMQDGPEAGQTVKHVHVHVLPRKAGDFHRNDSIYEELQKHDKEDFPASWRSEEEMAAEAAALRVYFQ
Gene ID
Swiss Prot
SynonymsFRA3B; AP3Aase
Calculated MW17kDa
Observed MW17kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesA-549, HeLa, Jurkat, 293T, Mouse liver, Mouse kidney
Cellular locationCytoplasm, Mitochondrion, Nucleus

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    ABclonal:Western blot - FHIT Rabbit pAb (A1196)}

    Western blot - FHIT Rabbit pAb (A1196)

    Western blot analysis of extracts of various cell lines, using FHIT antibody (A1196) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.

    * For research use only. Not for therapeutic or diagnostic purposes.

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